Figure 1From: Rapid detection of the CYP2A6*12 hybrid allele by Pyrosequencing®technology Alignment of DNA sequence for CYP2A6*1 and CYP2A6*12. Arrows indicate forward PCR and sequencing primers (5'-CTCCCTCTAACCACTCCCAC-3' and 5'-CACYCRCCTCCATCAG-3') and 3'-biotinylated reverse primer (5'-GCTCCCCGTTGCTGAATA-3'). The resulting 1274 bp PCR product is specific to CYP2A6*1 and CYP2A6*12 alleles. Exon 2 and 3 sequences are shown in bold, and the black boxes represent sequence differences between the two alleles that generate allele-specific peaks in the pyrosequencing reaction. Nucleotide numbering positions for both introns and exons are in reference to nucleotide 1, which is the A of the ATG start codon.Back to article page