Figure 2

Alleles of rs2794521 differentially bind transcription factor(s). EMSA was performed by using biotin-labeled probe A or probe G with HepG2 cell nuclear extract, with or without competition from unlabeled probe A or probe G. The GR binds to a GRE as a monomer and a dimer as previously reported, and the incubation of nuclear extract of HepG2 cells with probe A showed two kinds of DNA-protein complex with the GR motif oligonucleotide probes, which probably represent monomer (Band 2) and dimer (Band 1) of GR (Lane 2). The incubation of nuclear extract of HepG2 cells with probe G showed a large increase in the dimer (Lane 5, band 1), and a dramatic reduction in monomer (Lane 5, band 2). The experiments were repeated three times with similar results.