Table 2 Sequence of primers used in reverse transcriptase-polymerase chain reaction, 5' Rapid Amplification of cDNA ends (5'RACE) and (RT-PCR) and plasmid constructs

TIM3 RT-PCR primers

Forward

5'-tgctgctgctgctactacttaca-3'

Reverse

5'-aggttggccaaagagatgag-3'

5'RACE

First round forward

5'-gctggggtgtagaagcagggcagat-3'

First round reverse

5'-ccatcctaatacgactcactatagggc-3'

Nested PCR forward

5'-tgtctgtgtctctgctgggccatgt-3'

Nested PCR reverse

5'-actcactatagggctcgagcggc-3'

Plasmid constructs primers

Common reverse primer

5'-attatctcgagtggactgggtacttcttccaa

Forward primer +63 bp

5'-attatggtacctgactgtagacctggcagtgtt-3'

Forward primer -241 bp

5'-attatggtaccggacatgctccatttcaggt-3'

Forward primer -452 bp

5'-attatggtacctgaggcttatgctgggagtt-3'

Forward primer -914 bp

5'-attatggtaccaaaccactcagcctgtgagc-3'

Forward primer -1702 bp

5'-attatggtaccgccttgaccaagttcatgct-3'

Forward primer -2220 bp

5'-attatggtaccccagctccctacacacacaa-3'