Effect of the -206 G>A on the transcription activity of the SPINK5 promoter. COS-7 cells or Hela cells were transiently cotransfected with pGL3/-206G or pGL3/-206A and pRL-TK vector. The relative luciferase activity of the SPINK5 reporter constructs is represented as the ratio of the firefly luciferase activity to that of Renilla. Each experiment was conducted in triplicate for each sample, and the results are expressed as mean ± SD for three independent experiments. P value was determined by the Student's t test.