Transient co-transfections with the -244/+1163 C (A) or G (B) reporter constructs and members of the NF1 family of transcription factors in three different cell lines as indicated. Each transient transfection was made in triplicates and the results presented are mean differences of three independent experiments. C or G alleles with co-transfection of empty pCH-vector were set as controls. Both C and G allele controls are set to 100% and the effect of NF1 expression compared to either the C-Control (A) or G-Control (B). * indicated significant difference (P < 0.05) ± SEM.