Figure 4
From: Functional and genetic analysis in type 2 diabetes of Liver X receptor alleles – a cohort study
A. A putative NF1 binding site in the promoter region of the LXRB gene as suggested by TESS (underlined) [36]. The rs17373080 SNP is indicated in bold. B. EMSA with MIN6 whole cell extracts (WCE) and labelled oligonucleotides with the rs17373080 C and G variants, respectively. Lane 1: Free oligo C, 2: Oligo C + MIN6 WCE, 3–5: Oligo C + MIN6 WCE + increasing amounts of unlabeled NF1 consensus oligonucleotide, 6: Empty 7: Free oligo G, 8: Oligo G + MIN6 WCE, 9–11: Oligo G + MIN6 WCE + increasing amounts of unlabeled NF1 consensus oligonucleotide. C. EMSA with rs17373080 SNP oligo C and MIN6 whole cell extract or in vitro translated (IVT) NF1. Lane 1: Free oligo C, 2: Oligo C + MIN6 whole cell extract, 3: Oligo C + MIN6 whole cell extract + 250× unlabeled NF1 consensus oligonucleotide, 4: Oligo C + Empty IVT expression vector (vehicle) and 5–7: Oligo C + IVT NF1A1, NF1B and NF1X, respectively. The arrows indicate the positions of the NF1 interactions.