Figure 5From: Prevalence of pathogenetic MC4R mutations in Italian children with early Onset obesity, tall stature and familial history of obesity FACS Data. Fluorescence activated cell sorting analysis (FACS) of the MC4R Q307X mutation expressed in HEK-293. The total cell receptor expression levels were determined using permeabilized cells measuring both cell surface and intracellular protein expression. The cell surface expression levels were determined using non-permeabilized cells. Cell expression levels are presented relative to the wild type human-MC4R control. Total Q307X expression was 88%, surface expression was 31% and intracellular retention was about 57%.Back to article page