Figure 2

Effect of microsatellite D6S1276 polymorphism on BMP5 promoter activity. Luciferase reporter assays of the D6S1276 constructs were performed in MG63 (A), CH8 (B), and SW872 cells (C). Transcriptional activities are given as a percentage of the activity of the Promoter-Luciferase construct for each cell line. Data shown are the mean ± SD of at least 3 experiments done in triplicate. Black bars indicate results for haplotype I (rs9475431 T, rs9475430 T, and rs9475429 T). Checked bars indicate results for haplotype II (rs9475431 G, rs9475430 C, and rs9475429 A). *P < 0.05, ** P < 0.01, *** P < 0.005, **** P < 1.0 × 10^-6, NS is not significant (Student's t-test).