Table 2 Primers used for PCR amplification of the 11 exons and intron flanking regions of the ITGB1BP2 gene
From: Melusin gene (ITGB1BP2) nucleotide variations study in hypertensive and cardiopathic patients
Exon | Exon size (bp) | Primer Forward | Primer Reverse | Amplicon size (bp) |
|---|---|---|---|---|
1 | 63 | TCAACCAACGCTTCCATG | GGCTGTAAGATCACTCAG | 214 |
2 | 49 | AATTGGTCTGAGTGATCTT | CAGATCAGCTTAGCTTCC | 279 |
3 | 56 | GGAAGCTAAGCTGATCTG | CTAGTATAGATAAGGAGA | 168 |
4 | 145 | ATTTATGTACTTGGATTC | CTAGTTCCCAGTCCTCAA | 302 |
5 | 94 | AACTAAATCACCACCCTT | ATTGCCACTTCCACTAAA | 180 |
6 | 55 | TGAGTGGGGGGATGGATA | AGGGCCTTGGTAAACCTA | 227 |
7 | 70 | CCCAGGATGTGATGCT | GTAGGAAGATAATTCAACTGAATT | 304 |
8 | 99 | GCTATGAGGCTATGAGAC | TGGCTATGTGGTACAGAG | 273 |
9 | 113 | CACGTGCATCATGAGAAC | CTCTGCCTGTGAGATTGT | 254 |
10 | 62 | CAACCTGTGGATCATACC | TCCAGTCTAGCCGCTCTT | 222 |
11 | 227 | CTCCTCCTCAGGTCATAA | TCAACTGTCTGGTGTCAC | 256 |