From: Genetic effect of CysLTR2 polymorphisms on its mRNA synthesis and stabilization
Approach | Orientation | Location | Primer Sequence |
---|---|---|---|
RT-PCR | Â | Â | Â |
CysLTR2 | Forward | +29/+46 | 5'-CATCCATCTCCGTATCAG-3' |
 | Reverse | +734/+717 | 5'-GCCTTCCTGTGAGAAACC-3' |
GAPDH | Forward | Â | 5'-CGTCTTCACCATGGAGA-3' |
 | Reverse |  | 5'-CGGCCATCACGCCACAGTTT-3' |
Promoter construct | Forward | -1342/-1323 | 5'-TTTTCCTGCCTTGTTGTTGG-3' |
 | Reverse | +178/+159 | 5'-TGGACAACCCATTTCCCAAG-3' |
 | Nested, Forward | -1008/-987 | 5'-ATTTAGCTAGCCAAAACATTAAATGTAACTTAG-3' |
 | Nested, Reverse | -4/-30 | 5'-ATTATCTCGAGGGGTTAAAAAGAAACAGACACAAAAAG-3' |
UTR construct | Forward | +501/+517 | 5'-GGCTTCCTCAATAATGC-3' |
 | Reverse | +2787/+2770 | 5'-GGTTGACCAAATGCTGTG-3' |
 | Nested, Forward | +1042/+1062 | 5'-AAATAGCGGCCGCGGAGCTCTTAGATGAGACCTG-3' |
 | Nested, Reverse | +2410/+2385 | 5'-TTAATGCGGCCGCTTGGTAGGAAAGGACAGCTTTTATTC-3' |