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Table 1 Primer sequences used in this study

From: Genetic effect of CysLTR2 polymorphisms on its mRNA synthesis and stabilization

Approach

Orientation

Location

Primer Sequence

RT-PCR

   

CysLTR2

Forward

+29/+46

5'-CATCCATCTCCGTATCAG-3'

 

Reverse

+734/+717

5'-GCCTTCCTGTGAGAAACC-3'

GAPDH

Forward

 

5'-CGTCTTCACCATGGAGA-3'

 

Reverse

 

5'-CGGCCATCACGCCACAGTTT-3'

Promoter construct

Forward

-1342/-1323

5'-TTTTCCTGCCTTGTTGTTGG-3'

 

Reverse

+178/+159

5'-TGGACAACCCATTTCCCAAG-3'

 

Nested, Forward

-1008/-987

5'-ATTTAGCTAGCCAAAACATTAAATGTAACTTAG-3'

 

Nested, Reverse

-4/-30

5'-ATTATCTCGAGGGGTTAAAAAGAAACAGACACAAAAAG-3'

UTR construct

Forward

+501/+517

5'-GGCTTCCTCAATAATGC-3'

 

Reverse

+2787/+2770

5'-GGTTGACCAAATGCTGTG-3'

 

Nested, Forward

+1042/+1062

5'-AAATAGCGGCCGCGGAGCTCTTAGATGAGACCTG-3'

 

Nested, Reverse

+2410/+2385

5'-TTAATGCGGCCGCTTGGTAGGAAAGGACAGCTTTTATTC-3'

  1. Underlined nucleotide sequences are added to the 5' end of oligonucleotide primer for cloning. These cloning sites are Nhe I (GCTAGC), Xho I (CTCGAG), and Not I (GCGGCCGC), and they are preceded by A and T residues at random