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Figure 5 | BMC Medical Genetics

Figure 5

From: Genetic effect of CysLTR2 polymorphisms on its mRNA synthesis and stabilization

Figure 5

Flow cytometric analysis of the effects of the human CysLTR2 gene 3'-UTR on EGFP protein expression. The 3'-UTR region possessing each genotype (C or T) on c.2078 was inserted between the EGFP coding region and SV40 poly A site as described in the Methods. After each construct was co-transfected with the β-galactosidase expression vector as an internal control into 293 T cell lines, the expression levels of the EGFP protein were measured using a flow cytometer (A) and the fluorescence intensity of each construct was compared (B). The data are presented as the mean ± SEM of relative FL1 values (the ratio of EGFP--CysLTR2 3'-UTR fusion constructs to naive pEGFP-N1 vector) of six independent experiments. The P value was calculated using a Mann--Whitney U-test.

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