Figure 3

Promoter ( c .-- 819T>G ) activity of the human CysLTR2 gene. The 293 T cells expressed CysLTR2, which increased with 24 h PMA/ionomycin stimulation (A) The promoter region of CysLTR2 having c.--819T or c.--819G was inserted into the pGL3 basic vector and the constructs and β-galactosidase expression vector were co-transfected into 293 T cells by lipofection. Twenty-four hours after transfection, the transfected cells (2.5 × 10⁵/ml) were stimulated with 10 ng/ml of PMA and 2 mM of ionomycin for 24 h. Luciferase activities were measure by luminometer and normalized by β-galactosidase activities as internal controls. (B) The data are presented as the mean ± SEM of normalized luciferase activities (the ratio of luciferase activities to β-galactosidase activities) of six independent experiments. *P < 0.01 calculated using a Mann--Whitney U-test for comparing luciferase activities between PMA/ionomycin-treated c.--819G and c.--819T promoter. † P < 0.05 calculated using Wilcoxon's signed-ranks test for comparing luciferase activities of PMA/ionomycin-treated and untreated c.819T promoter.