Table 3 Primers used for construct amplification of the IVS7 variant

IVS 5-3' BamHI

5'-GATGGGGATCCCAGCACCATCACACTGTTTGAG-3'

IVS6-5' XhoI

5'-CGGCGCTCGAGGAAATCATCACAAATGCCAAG-3'

Segment 2

IVS 6-3'

5'-CGCTCGAGGATGTTGTTCATGTAATAAA-3'

IVS 7-5' HindIII

5'-CGAAGCTTCCAACTGTGACAGCCCACAA-3'

Segment 3

IVS 7-3' HindIII

5'-GCAAGCTTGTGAGGCGTCATTAACTTGT-3'

IVS 8-5' PvuI

5'-GCCGATCGTTATTCCTGTTAGTTAGCAA-3'

Segment 4

IVS 8-3' PvuI

5'-GCCGATCGGCAGAGGGGTCTCAGAATCC-3'

IVS 9-5'

5'-CCTTCTGCCATTATCTCATTCTTG-3'