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Figure 3 | BMC Medical Genetics

Figure 3

From: Mutation spectrum of 122 hemophilia A families from Taiwanese population by LD-PCR, DHPLC, multiplex PCR and evaluating the clinical application of HRM

Figure 3

Comparison of the results of different HRM primers. a) and c) are HRM plots for exon 14-5 with first-designed primer. We are unable to see the grouping in the normalized, temperature-shifted plot. So we redesigned the primer pairs by LightCycler Probe Design Software 2.0. The result is shown in b) and d). The carrier and the patient are easy to be distinguished in the latter.

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BMC Medical Genetics

ISSN: 1471-2350

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