The FH mutation database: an online database of fumarate hydratase mutations involved in the MCUL (HLRCC) tumor syndrome and congenital fumarase deficiency
© Bayley et al; licensee BioMed Central Ltd. 2008
Received: 11 December 2007
Accepted: 25 March 2008
Published: 25 March 2008
Fumarate hydratase (HGNC approved gene symbol – FH), also known as fumarase, is an enzyme of the tricarboxylic acid (TCA) cycle, involved in fundamental cellular energy production. First described by Zinn et al in 1986, deficiency of FH results in early onset, severe encephalopathy. In 2002, the Multiple Leiomyoma Consortium identified heterozygous germline mutations of FH in patients with multiple cutaneous and uterine leiomyomas, (MCUL: OMIM 150800). In some families renal cell cancer also forms a component of the complex and as such has been described as hereditary leiomyomatosis and renal cell cancer (HLRCC: OMIM 605839). The identification of FH as a tumor suppressor was an unexpected finding and following the identification of subunits of succinate dehydrogenase in 2000 and 2001, was only the second description of the involvement of an enzyme of intermediary metabolism in tumorigenesis.
The FH mutation database is a part of the TCA cycle gene mutation database (formerly the succinate dehydrogenase gene mutation database) and is based on the Leiden Open (source) Variation Database (LOVD) system. The variants included in the database were derived from the published literature and annotated to conform to current mutation nomenclature. The FH database applies HGVS nomenclature guidelines, and will assist researchers in applying these guidelines when directly submitting new sequence variants online. Since the first molecular characterization of an FH mutation by Bourgeron et al in 1994, a series of reports of both FH deficiency patients and patients with MCUL/HLRRC have described 107 variants, of which 93 are thought to be pathogenic. The most common type of mutation is missense (57%), followed by frameshifts & nonsense (27%), and diverse deletions, insertions and duplications. Here we introduce an online database detailing all reported FH sequence variants.
The FH mutation database strives to systematically unify all current genetic knowledge of FH variants. We believe that this knowledge will assist clinical geneticists and treating physicians when advising patients and their families, will provide a rapid and convenient resource for research scientists, and may eventually assist in gaining novel insights into FH and its related clinical syndromes.
Recently two proteins involved in the tricarboxcylic acid (TCA) cycle have been shown to be tumor suppressors. Fumarate hydratase (FH) (also known as fumarase) and succinate dehydrogenase (SDH), which also plays a role in oxidative phosphorylation, are enzymes involved in fundamental processes of energy production. Deficiencies of FH and SDH(A) generally result in early-onset, severe encephalopathy. The first description of fumarate hydratase deficiency was in 1986 by Zinn et al , followed in 1994 by the first molecular characterization of an FH mutation by Bourgeron et al . In 2002, the Multiple Leiomyoma Consortium identified FH as the tumor suppressor gene responsible for MCUL/HLRCC .
The identification of these genes as tumor suppressors was an entirely unexpected finding and demonstrated for the first time the involvement of proteins of intermediary metabolism in tumorigenesis. Mutations have been identified in both the gene encoding fumarate hydratase and three of the four genes encoding succinate dehydrogenase, subunits B, C and D (SDHB, -C and -D) [4–6], while no cancer-related mutations have yet been reported in SDHA. Germline mutations in FH predispose individuals to multiple cutaneous leiomyomas, uterine leiomyomas and in some families renal cell cancer (HLRCC) , whereas mutations in SDH cause hereditary paragangliomas and pheochromocytomas (HPGL) . Both of these cancer syndromes are inherited in an autosomal dominant manner. Despite the closely related function of FH and SDH proteins, the tumor spectra in HPGL and HLRCC show little overlap, indicating that although biochemically related, the mitogenic stimulus leading to tumor formation must be cell specific.
The principal phenotype of the FH-associated tumor syndrome is skin leiomyoma. These are typically sensitive to cold or abrasion, appear to be more common in women than men, developing between the second and fourth decades as intradermal papules or nodules of up to 20 mm in diameter, with a disseminated or segmental distribution. Germline FH mutations have been identified in the vast majority of patients with multiple skin leiomyomas, and the relatives of probands have often been subsequently diagnosed with skin leiomyomas, suggesting that many more cases are currently going unrecognized. Female FH mutation carriers are also at high risk of early-onset uterine fibroids that frequently require hysterectomy. Certain FH mutations have also been associated with uterine fibroids without skin leiomyomas . Although uterine fibroids are the most common tumors in women during their reproductive years, FH mutations do not appear to play a major role in non-syndromic cases . Although not always present in the FH syndrome, aggressive renal cell carcinomas of two unusual types, type II papillary and collecting duct morphology, also occur in certain families. Both frequently present with metastatic disease before the age of fifty and are associated with high mortality. A single case has also been reported with both papillary and conventional clear cell renal carcinoma, both tumors displaying loss of the wild type FH allele and immunostaining . Recently Merino et al described a distinctive histological feature of these tumors, a characteristically large nucleus with a very prominent inclusion-like orangiophilic or eosinophilic nucleolus, surrounded by a clear halo .
Leiomyosarcomas also occur in association with FH mutations but appear to be rare in FH mutation carriers although a few cases, predominantly of Finnish origin, have been described [13, 14]. Other reported pathology associated with FH mutations include cerebral cavernomas  and Leydig cell tumors  and ovarian mucinous cystadenoma .
A number of biochemical studies have shown that FH-deficient cells and tumors accumulate both fumarate and succinate, while SDH-deficient tumors mainly accumulate succinate [18–20]. Accumulation of these metabolic intermediates may affect other biochemical processes in the cell, and considerable evidence indicates that the inhibition of HIF prolyl hydroxylase leads to the over-expression of the transcription factor, hypoxia-inducible factor 1α (HIF1α) . HIF1α targets, including vascular endothelial growth factor (VEGF), erythropoietin (EPO), and glucose transporter1 (GLUT1), show activation on accumulation of TCA metabolites, and contribute to an increase in microvessel density [18, 20, 22–26]. This phenomenon has been described as "pseudo-hypoxia" and shows striking similarities with von Hippel-Lindau (VHL) syndrome . In normoxia, HIF1α is hydroxylated at critical proline residues by a HIF prolyl hydroxylase, and targeted for degradation by an E3-ubiquitin ligase complex that includes the VHL protein. In VHL disease, mutations in VHL inhibit this process and lead to the stabilization of HIF1α. Phenotypically, both HPGL and VHL patients may develop pheochromocytomas, and the clear cell renal carcinomas that are a major feature of VHL disease  have recently been reported in a HPGL family .
Construction and content
The FH mutation database  is based on the recently described Leiden Open (source) Variation Database (LOVD) system  which, under the auspices of the Human Genome Variation Society, is rapidly becoming the standard for gene-specific databases. The LOVD system was designed to be flexible so that it could be easily adapted to the needs of a wide range of locus specific databases. Additional flexibility comes from the platform-independent design and the use of PHP and MySQL open source software. The design is gene-centered and modular, and focuses on the collection and display of DNA sequence variations. The LOVD platform is also easily extendable to include summary clinical data. In addition, all database contents may be downloaded in flat text format and imported into a spreadsheet program for further analysis. The LOVD database software is now in a new version, LOVD2, which has many additional features.
Researchers interested in contributing sequence variants to the database will be asked to register, (submitters can access and edit their personal data at any time), after which they can contribute the variant online. During submission researchers are asked to provide those data that are deemed absolutely essential for mutation databases by the Human Genome Organization Mutation Database Initiative. These include a patient ID, an exact molecular description of the variant (DNA-level), and details about the source of the material and detection method used. Newly submitted variants are given a unique identifier, as recommended by Claustres et al . After the curator's approval the new variant is added to the database and all connected web pages are updated instantly. Currently (Jan, 2008) all database content is derived from published, peer-reviewed literature. We anticipate that directly submitted content will become an increasing proportion in the future. As the submitted data are in the form of a submitter deduced mutation description, the curators can only check the feasibility and consistency of the description and not any original data.
DNA variation in populations is generally benign and as such should be termed a 'variant' or 'SNP'. The term 'mutation' is reserved for cases were there is a reasonable suspicion that the variant is deleterious. DNA variants submitted to the FH database should be described in accordance with the recommendations of the Human Genome Variation Society (HGVS), update August 2004, and it is strongly recommended that authors consult the most up-to-date nomenclature guidelines, which can be found online at the HGVS website. Unfortunately the mutation nomenclature currently used in the field of FH research is rarely in agreement with HGVS guidelines. Reports contain a variety of annotations, and many are based on the annotation of the cytosolic protein, the function of which is currently poorly defined . One of the aims of the HGVS is to establish a consistent nomenclature across all human genes. The description of FH protein variants based on the cytosolic protein is often accompanied by the use of a truncated transcript to describe cDNA variants. Other authors use the cytosolic protein numbering system, while applying the full-length coding transcript to describe cDNA variants. This results in the loss of the simple approximation of 3 to 1 between the numbering. The HGVS-recommended cDNA numbering starts from the first ATG of the full coding sequence. Protein reference sequences should represent the primary translation product, not a processed mature protein, and thus include any signal peptide sequences Currently many variants are present in the literature under several titles, and the correct identification of intron variants is especially problematic. No reliable overview of known FH variation can be achieved without a consistent nomenclature, which includes the appropriate numbering systems at both the DNA and protein level. We now recommend that all authors implement the recommendations of the HGVS when describing future FH variants.
The FH mutation database will accept all variants and if necessary assist in assigning the correct nomenclature. In light of the inconsistency of the recent FH mutation nomenclature, variants have been reassigned, where necessary, and an extra column has been generated clearly stating the originally reported description(s) of the mutation at the protein level, and where equivocal also at the cDNA level. Inclusion of sequence variants in the FH mutation database does not imply that there is convincing evidence for pathogenicity. Please refer to the disclaimer on the website.
Within the FH database, all variants that disrupt the reading frame, affect highly conserved residues (conserved by sequence alignment in at least Saccharomyces cerevisiae) or disrupt the consensus donor or acceptor splice sites (GT-AG), and are not found in healthy controls, should be suspected as potentially pathogenic. Non-conserved missense variants and potential splice site mutations that do not disrupt the consensus splice sites, are considered to be SNPs or rare variants of uncertain significance. Ideally, several lines of evidence should support publications which present mutations as "pathogenic", including screening DNA from a panel of 50–200 healthy individuals, describing the nature of the amino acid substitution (conservative/non-conservative) and the significance of the position in the protein (evolutionarily conservation or known functional domain). It is important to note whether the mutation has previously been reported, if it has been found in several families, and if it segregates with the disease within the family.
Many FH variants have been analysed for effects on enzymatic function, and this data has, in many cases, allowed the classification of missense variants as pathogenic. In addition a SIFT (Sorting Intolerant From Tolerant) analysis  of missense FH variants has been included. A high score (above 0.05) indicates that an amino acid is poorly conserved (alignment of 337 Uniprot proteins), indicating caution in assignment of function to the variant.
However none of these factors can be seen as definitive and each variant must be considered on its merits. Unfortunately, most mutations are currently reported without this accompanying analysis, and many have been identified in a single case or family. Thus caution should be exercised when attempting to derive clinically relevant information from the database, and users must carefully weigh all the evidence in the database and any additional data.
The FH gene is very highly conserved, showing 67% protein identity between humans and S. cerevisiae with the few coding SNPs described in dbSNP present in the HapMap population as very rare alleles (the minor allele being undetected). Thus, current knowledge suggests that most missense changes will be pathogenic. Polymorphisms, including intron variants, synonymous (silent) variants, nonsynonymous missense variants found in a healthy control panel and potential non-consensus splice site mutations but without evidence for transcript rearrangements are included in the database as such unless accompanied by clear evidence of pathogenic potential.
An abbreviated description of the FH related syndrome is given under "disease". Refer to the database notes for an explanation of these abbreviations. Further information can be found under "remarks", including the country of origin of the patient or study, together with other details such as the number of healthy controls tested for the variant, and any other supporting evidence.
That conservation is not always a good predictor of function is seen with Lys467Arg (K424R) missense variant. This amino acid is relatively poorly conserved, present in E. coli, but variable in many other species. The SIFT score is 0.17, indicating that this is a tolerated variant, and should not affect protein function. Other species variants include Asn, Ala, Met, and Glu, all of which are also predicted by SIFT analysis to be tolerated changes. However, Ylisaukko-oja et al recently demonstrated that this variant has less than half the activity of the wt variant . Context may also be important for the function of a variant, a case in point being Glu362Gln (Glu319Gln), the very first FH mutation, described by Bourgeron in 1994 . Two siblings with profound fumarase deficiency were found to be homozygous for this mutation, showing only residual fumarase activity of less than 0.5% of controls. The glutamine (Gln) variant residue is the only change predicted by SIFT analysis to be tolerated. It can be speculated that any other change in this highly conserved distal region of exon 7 would have resulted in such a complete loss of fumarase activity as to be incompatible with life.
Renal cell cancer (RCC) associated mutations occur throughout the gene with the exception of exons 1 and 6, though there are perhaps fewer RCC-associated mutations in exon 7 than one might expect (Fig 4). The Arg233His (R190H) mutation is the most commonly described FH variant, so it is unsurprising this residue should be mutated in a few cases of RCC. However, arginine 233 is also independently affected, with less common variants (Arg233Cys, Arg233Leu) and all changes have been associated with RCC. This residue resides in the A-site which may be the main catalytically active site . Previously Alam et al  speculated that truncating mutations may be over-represented in renal cancer families. Within the current database, truncating and missense mutations are equally represented (11 vs. 11) in renal cell cancer patients (Fig 4). This still represents relatively more truncating mutations associated with RCC than in the overall database, but the current trend seems to indicate that the initial imbalance was an artefact of the limited numbers of variants then known. Whether truncating mutations are more penetrant, leading to more cases of RCC than the missense variants, is currently unclear from the published clinical data.
A number of FH missense mutations have been reported to reduce FH activity below that seen for truncating mutations, indicating a dominant negative action . A second study failed to confirm differential activities of truncating and missense mutations on FH enzyme activity in lymphoblastoid cell lines from HLRCC patients . However, Lorenzato et al recently formally demonstrated the dominant negative action of the Arg233His (R190H) mutation . For a more complete functional analysis of FH missense mutations see Alam et al [36, 37].
As described above, patients with mutations of FH display a range of pathologies, the most profound, FH deficiency, related to mutation of both FH alleles. We have previously noted [3, 37] the tendency of FH deficiency-associated mutations to occur later in the gene (Fig 4) and to be less likely to include mutations leading to complete loss of protein function. While several cases of FH deficiency have been reported together with truncating mutations, the other affected allele tended to carry a missense mutation. The only case carrying two profound mutations was reported by Coughlin et al . This patient (F1430) had only 2% residual FH enzyme activity, but did not show the most serious of the known clinical manifestations. Even the mutations in this patient may have been attenuated by the fact that while one allele was affected by a 74 bp deletion in exon 2, the other truncating mutation occurred very late in the protein at the end of exon 10, leading to the loss of the last 10 amino acid residues. These data suggest that some residual enzyme activity is required if an embryo is to develop to term.
Here we present an online database of FH gene variants that provides the only complete and up-to-date overview of all reported disease-related mutations.
Clearly, considering the geographical distribution of the studies to date, only a fraction of all FH mutations associated with MCUL/HLRCC have yet been identified. While no unequivocal genotype-phenotype correlations associated with certain types of mutations or location in the gene have emerged so far , increasing knowledge of FH mutations may yet provide insight into sub-phenotypes or FH protein function. Importantly, every FH mutation found in an MCUL/HLRCC patient increases confidence in the pathogenic role of that variant, improving the accuracy of clinical genetic counselling. Equally it remains extremely important to test missense variants in sufficient, ethnically matched, healthy controls to aid in identifying SNPs in the coding sequence, which may currently be incorrectly classified.
We hope that the FH mutation database, which strives to systematically unify all current genetic knowledge of FH variants, will increase the confidence of clinical geneticists and treating physicians when advising patients and their families, will provide a convenient resource for research scientists, and may eventually assist in gaining novel insights into FH and its related clinical syndromes.
Availability and requirements
The FH mutation database; http://chromium.liacs.nl/LOVD2/SDH/home.php?select_db=FH is freely accessible and all researchers may submit new sequence variants online (after registration – to collect contact information for reference purposes and clarification of submitted details, as well as to assign a login name and password).
- Zinn AB, Kerr DS, Hoppel CL: Fumarase Deficiency - A New Cause of Mitochondrial Encephalomyopathy. New England Journal of Medicine. 1986, 315: 469-475.View ArticlePubMedGoogle Scholar
- Bourgeron T, Chretien D, Poggibach J, Doonan S, Rabier D, Letouze P, Munnich A, Rotig A, Landrieu P, Rustin P: Mutation of the Fumarase Gene in 2 Siblings with Progressive Encephalopathy and Fumarase Deficiency. Journal of Clinical Investigation. 1994, 93: 2514-2518. 10.1172/JCI117261.View ArticlePubMedPubMed CentralGoogle Scholar
- Tomlinson IPM, Alam NA, Rowan AJ, Barclay E, Jaeger EEM, Kelsell D, Leigh I, Gorman P, Lamlum H, Rahman S, Roylance RR, Olpin S, Bevan S, Barker K, Hearle N, Houlston RS, Kiuru M, Lehtonen R, Karhu A, Vilkki S, Laiho P, Eklund C, Vierimaa O, Aittomaki K, Hietala M, Sistonen P, Paetau A, Salovaara R, Herva R, Launonen V, Aaltonen LA: Germline mutations in FH predispose to dominantly inherited uterine fibroids, skin leiomyomata and papillary renal cell cancer. Nature Genetics. 2002, 30: 406-410. 10.1038/ng849.View ArticlePubMedGoogle Scholar
- Niemann S, Muller U: Mutations in SDHC cause autosomal dominant paraganglioma, type 3. Nature Genetics. 2000, 26: 268-270. 10.1038/81551.View ArticlePubMedGoogle Scholar
- Baysal BE, Ferrell RE, Willett-Brozick JE, Lawrence EC, Myssiorek D, Bosch A, van der Mey A, Taschner PEM, Rubinstein WS, Myers EN, Richard CW, Cornelisse CJ, Devilee P, Devlin B: Mutations in SDHD, a mitochondrial complex II gene, in hereditary paraganglioma. Science. 2000, 287: 848-851. 10.1126/science.287.5454.848.View ArticlePubMedGoogle Scholar
- Astuti D, Latif F, Dallol A, Dahia PLM, Douglas F, George E, Skoldberg F, Husebye ES, Eng C, Maher ER: Gene mutations in the succinate dehydrogenase subunit SDHB cause susceptibility to familial pheochromocytoma and to familial paraganglioma. American Journal of Human Genetics. 2001, 69: 49-54. 10.1086/321282.View ArticlePubMedPubMed CentralGoogle Scholar
- Sudarshan S, Linehan WM, Neckers L: HIF and fumarate hydratase in renal cancer. British Journal of Cancer. 2007, 96: 403-407. 10.1038/sj.bjc.6603547.View ArticlePubMedPubMed CentralGoogle Scholar
- Maher ER, Eng C: The pressure rises: update on the genetics of phaeochromocytoma. Human Molecular Genetics. 2002, 11: 2347-2354. 10.1093/hmg/11.20.2347.View ArticlePubMedGoogle Scholar
- Alam NA, Barclay E, Rowan AJ, Tyrer JP, Calonje E, Manek S, Kelsell D, Leigh I, Olpin S, Tomlinson IPM: Clinical features of multiple cutaneous and uterine leiomyomatosis - An underdiagnosed tumor syndrome. Archives of Dermatology. 2005, 141: 199-206. 10.1001/archderm.141.2.199.PubMedGoogle Scholar
- Lehtonen R, Kiuru M, Vanharanta S, Sjoberg J, Aaltonen LM, Aittomaki K, Arola J, Butzow R, Eng C, Husgafvel-Pursiainen K, Isola J, Jarvinen H, Koivisto P, Mecklin JP, Peltomaki P, Salovaara R, Wasenius VM, Karhu A, Launonen V, Nupponen NN, Aaltonen LA: Biallelic inactivation of fumarate hydratase (FH) occurs in nonsyndromic uterine leiomyomas but is rare in other tumors. American Journal of Pathology. 2004, 164: 17-22.View ArticlePubMedPubMed CentralGoogle Scholar
- Lehtonen HJ, Blanco I, Piulats JM, Herva R, Launonen V, Aaltonen LA: Conventional renal cancer in a patient with fumarate hydratase mutation. Human Pathology. 2007, 38: 793-796. 10.1016/j.humpath.2006.10.011.View ArticlePubMedGoogle Scholar
- Merino MJ, Torres-Cabala C, Pinto P, Linehan WM: The morphologic spectrum of kidney tumors in hereditary leiomyomatosis and renal cell carcinoma (HLRCC) syndrome. Am J Surg Pathol. 2007, 31: 1578-1585.View ArticlePubMedGoogle Scholar
- Ylisaukko-oja SK, Kiuru M, Lehtonen HJ, Lehtonen R, Pukkala E, Arola J, Launonen V, Aaltonen LA: Analysis of fumarate hydratase mutations in a population-based series of early onset uterine leiomyosarcoma patients. International Journal of Cancer. 2006, 119: 283-287. 10.1002/ijc.21798.View ArticlePubMedGoogle Scholar
- Kiuru M, Lehtonen R, Arola J, Salovaara R, Jarvinen H, Aittomaki K, Sjoberg J, Visakorpi T, Knuutila S, Isola J, Delahunt B, Herva R, Launonen V, Karhu A, Aaltonen LA: Few FH mutations in sporadic counterparts of tumor types observed in hereditary Leiomyomatosis and renal cell cancer families. Cancer Research. 2002, 62: 4554-4557.PubMedGoogle Scholar
- Campione E, Terrinoni A, Orlandi A, Codispoti A, Melino G, Bianchi L, Mazzotta A, Garaci FG, Ludovici A, Chimenti S: Cerebral cavernomas in a family with multiple cutaneous and uterine leiomyomas associated with a new mutation in the fumarate hydratase gene. J Invest Dermatol. 2007, 127: 2271-2273. 10.1038/sj.jid.5700851.View ArticlePubMedGoogle Scholar
- Carvajal-Carmona LG, Alam NA, Pollard PJ, Jones AM, Barclay E, Wortham N, Pignatelli M, Freeman A, Pomplun S, Ellis I, Poulsom R, El Bahrawy MA, Berney DM, Tomlinson IPM: Adult Leydig cell tumors of the testis caused by germline fumarate hydratase mutations. Journal of Clinical Endocrinology and Metabolism. 2006, 91: 3071-3075. 10.1210/jc.2006-0183.View ArticlePubMedGoogle Scholar
- Ylisaukko-oja SK, Cybulski C, Lehtonen R, Kiuru M, Matyjasik J, Szymanska A, Szymanska-Pasternak J, Dyrskjot L, Butzow R, Orntoft TF, Launonen V, Lubinski J, Aaltonen LA: Germline fumarate hydratase mutations in patients with ovarian mucinous cystadenoma. European Journal of Human Genetics. 2006, 14: 880-883. 10.1038/sj.ejhg.5201630.View ArticlePubMedGoogle Scholar
- Selak MA, Armour SM, MacKenzie ED, Boulahbel H, Watson DG, Mansfield KD, Pan Y, Simon MC, Thompson CB, Gottlieb E: Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-alpha prolyl hydroxylase. Cancer Cell. 2005, 7: 77-85. 10.1016/j.ccr.2004.11.022.View ArticlePubMedGoogle Scholar
- Pollard PJ, Briere JJ, Alam NA, Barwell J, Barclay E, Wortham NC, Hunt T, Mitchell M, Olpin S, Moat SJ, Hargreaves IP, Heales SJ, Chung YL, Griffiths JR, Dalgleish A, McGrath JA, Gleeson MJ, Hodgson SV, Poulsom R, Rustin P, Tomlinson IPM: Accumulation of Krebs cycle intermediates and over-expression of HIF1 alpha in tumours which result from germline FH and SDH mutations. Human Molecular Genetics. 2005, 14: 2231-2239. 10.1093/hmg/ddi227.View ArticlePubMedGoogle Scholar
- Isaacs JS, Jung YJ, Mole DR, Lee S, Torres-Cabala C, Chung YL, Merino M, Trepel J, Zbar B, Toro J, Ratcliffe PJ, Linehan WM, Neckers L: HIF overexpression correlates with biallelic loss of fumarate hydratase in renal cancer: Novel role of fumarate in regulation of HIF stability. Cancer Cell. 2005, 8: 143-153. 10.1016/j.ccr.2005.06.017.View ArticlePubMedGoogle Scholar
- Hirota K, Semenza GL: Regulation of hypoxia-inducible factor 1 by prolyl and asparaginyl hydroxylases. Biochemical and Biophysical Research Communications. 2005, 338: 610-616. 10.1016/j.bbrc.2005.08.193.View ArticlePubMedGoogle Scholar
- Gimenez-Roqueplo AP, Favier J, Rustin P, Mourad JJ, Plouin PF, Corvol P, Rotig A, Jeunemaitre X: The R22X mutation of the SDHD gene in hereditary paraganglioma abolishes the enzymatic activity of complex II in the mitochondrial respiratory chain and activates the hypoxia pathway. Am J Hum Genet. 2001, 69: 1186-1197. 10.1086/324413.View ArticlePubMedPubMed CentralGoogle Scholar
- Gimenez-Roqueplo AP, Favier J, Rustin P, Rieubland C, Kerlan V, Plouin PF, Rotig A, Jeunemaitre X: Functional consequences of a SDHB gene mutation in an apparently sporadic pheochromocytoma. J Clin Endocrinol Metab. 2002, 87: 4771-4774. 10.1210/jc.2002-020525.View ArticlePubMedGoogle Scholar
- Koivunen P, Hirsila M, Remes AM, Hassinen IE, Kivirikko KI, Myllyharju J: Inhibition of hypoxia-inducible factor (HIF) hydroxylases by citric acid cycle intermediates - Possible links between cell metabolism and stabilization of HIF. Journal of Biological Chemistry. 2007, 282: 4524-4532. 10.1074/jbc.M610415200.View ArticlePubMedGoogle Scholar
- MacKenzie ED, Selak MA, Tennant DA, Payne LJ, Crosby S, Frederiksen CM, Watson DG, Gottlieb E: Cell-permeating alpha-ketoglutarate derivatives alleviate pseudohypoxia in succinate dehydrogenase-deficient cells. Molecular and Cellular Biology. 2007, 27: 3282-3289. 10.1128/MCB.01927-06.View ArticlePubMedPubMed CentralGoogle Scholar
- Pollard P, Wortham N, Barclay E, Alam A, Elia G, Manek S, Poulsom R, Tomlinson I: Evidence of increased microvessel density and activation of the hypoxia pathway in turnours from the hereditary leiomyomatosis and renal cell cancer syndrome. Journal of Pathology. 2005, 205: 41-49. 10.1002/path.1686.View ArticlePubMedGoogle Scholar
- Kim WY, Kaelin WG: Role of VHL gene mutation in human cancer. Journal of Clinical Oncology. 2004, 22: 4991-5004. 10.1200/JCO.2004.05.061.View ArticlePubMedGoogle Scholar
- Clifford SC, Prowse AH, Affara NA, Buys CHCM, Maher ER: Inactivation of the von Hippel-Lindau (VHL) tumour suppressor gene and allelic losses at chromosome arm 3p in primary renal cell carcinoma: Evidence for a VHL-independent pathway in clear cell renal tumourigenesis. Genes Chromosomes & Cancer. 1998, 22: 200-209. 10.1002/(SICI)1098-2264(199807)22:3<200::AID-GCC5>3.0.CO;2-#.View ArticleGoogle Scholar
- Vanharanta S, Buchta M, McWhinney SR, Virta SK, Peczkowska M, Morrison CD, Lehtonen R, Januszewicz A, Jarvinen H, Juhola M, Mecklin JP, Pukkala E, Herva R, Kiuru M, Nupponen NN, Aaltonen LA, Neumann HPH, Eng C: Early-onset renal cell carcinoma as a novel extraparaganglial component of SDHB-associated heritable paraganglioma. American Journal of Human Genetics. 2004, 74: 153-159. 10.1086/381054.View ArticlePubMedGoogle Scholar
- Bayley JP, Launonen V, Tomlinson IPM: The TCAC Mutation Database. Fumarate Hydratase. 2008, [http://chromium.liacs.nl/LOVD2/SDH/home.php?select_db=FH]Google Scholar
- Fokkema IFAC, den Dunnen JT, Taschner PEM: LOVD: Easy creation of a locus-specific sequence variation database using an "LSDB-in-a-Box" approach. Human Mutation. 2005, 26: 63-68. 10.1002/humu.20201.View ArticlePubMedGoogle Scholar
- Claustres M, Horaitis O, Vanevski M, Cotton RGH: Time for a unified system of mutation description and reporting: A review of locus-specific mutation Databases. Genome Research. 2002, 12: 680-688. 10.1101/gr.217702.View ArticlePubMedGoogle Scholar
- Singh B, Gupta RS: Mitochondrial import of human and yeast fumarase in live mammalian cells: Retrograde translocation of the yeast enzyme is mainly caused by its poor targeting sequence. Biochemical and Biophysical Research Communications. 2006, 346: 911-918. 10.1016/j.bbrc.2006.05.188.View ArticlePubMedGoogle Scholar
- Ng PC, Henikoff S: SIFT: Predicting amino acid changes that affect protein function. Nucleic Acids Res. 2003, 31: 3812-3814. 10.1093/nar/gkg509.View ArticlePubMedPubMed CentralGoogle Scholar
- Weaver T, Lees M, Banaszak L: Mutations of fumarase that distinguish between the active site and a nearby dicarboxylic acid binding site. Protein Sci. 1997, 6: 834-842.View ArticlePubMedPubMed CentralGoogle Scholar
- Alam NA, Olpin S, Rowan A, Kelsell D, Leigh IM, Tomlinson IPM, Weaver T: Missense mutations in fumarate hydratase in multiple cutaneous and uterine leiomyomatosis and renal cell cancer. Journal of Molecular Diagnostics. 2005, 7: 437-443.View ArticlePubMedPubMed CentralGoogle Scholar
- Alam NA, Rowan AJ, Wortham NC, Pollard PJ, Mitchell M, Tyrer JP, Barclay E, Calonje E, Manek S, Adams SJ, Bowers PW, Burrows NP, Charles-Holmes R, Cook LJ, Daly BM, Ford GP, Fuller LC, Hadfield-Jones SE, Hardwick N, Highet AS, Keefe M, MacDonald-Hull SP, Potts EDA, Crone M, Wilkinson S, Camacho-Martinez F, Jablonska S, Ratnavel R, MacDonald A, Mann RJ, Grice K, Guillet G, Lewis-Jones MS, McGrath H, Seukeran DC, Morrison PJ, Fleming S, Rahman S, Kelsell D, Leigh I, Olpin S, Tomlinson IPM: Genetic and functional analyses of FH mutations in multiple cutaneous and uterine leiomyomatosis, hereditary leiomyomatosis and renal cancer, and fumarate hydratase deficiency. Human Molecular Genetics. 2003, 12: 1241-1252. 10.1093/hmg/ddg148.View ArticlePubMedGoogle Scholar
- Pithukpakorn M, Wei MH, Toure O, Steinbach PJ, Glenn GM, Zbar B, Linehan WM, Toro JR: Fumarate hydratase enzyme activity in lymphoblastoid cells and fibroblasts of individuals in families with hereditary leiomyomatosis and renal cell cancer. Journal of Medical Genetics. 2006, 43: 755-762. 10.1136/jmg.2006.041087.View ArticlePubMedPubMed CentralGoogle Scholar
- Lorenzato A, Olivero M, Perro M, Briere JJ, Rustin P, Di Renzo MF: A cancer-predisposing "hot spot" mutation of the fumarase gene creates a dominant negative protein. Int J Cancer. 2008, 122: 947-951. 10.1002/ijc.23209.View ArticlePubMedGoogle Scholar
- Coughlin EM, Christensen E, Kunz PL, Krishnamoorthy KS, Walker V, Dennis NR, Chalmers RA, Elpeleg ON, Whelan D, Pollitt RJ, Ramesh V, Mandell R, Shih VE: Molecular analysis and prenatal diagnosis of human fumarase deficiency. Molecular Genetics and Metabolism. 1998, 63: 254-262. 10.1006/mgme.1998.2684.View ArticlePubMedGoogle Scholar
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