Table 1 Primers for PCR and sequencing
Deletion | PCR primers | Fragment size* | Annealing temperature | Sequencing primers |
|---|---|---|---|---|
Promoter – exon1 | F: gtccgaggaaggtcacagaa R: cagcacacaaatgaggtggt | 3.5 kb | 60 | R: cagcacacaaatgaggtggt |
Exon5 | F: gtggtctcggccatccatcc R: tctgcaagccgcctgcaccg | 1.3 kb | 72** | R: tctgcaagccgcctgcaccg |
Exon7 – 8 | F: tcctccttcctctctctggc R: gctgcaggcaggggcgacgc | 3 kb | 63 | R: aaagccaggcacggtggctc |
Exon9 – 14 | F: ggctacaagtgccagtgtga R: agctgacctttagcctgacg | 2 kb | 59 | F: tttttgagacagagtctca R: aaagtccaaaatcaggcc |
Exon 13 – 15 | F: tctccttatccacttgtgtgtctag R: gctttggtcttctctgtctttgaat | 8 kb | 58 | F: tagccaggtgtggtggcagg R: ctgggagtagctaggactgc |