Table 1 Oligonucleotide primers used in the study.
From: Identification of novel functional sequence variants in the gene for peptidase inhibitor 3
Primer Code | Sequencea | Purpose | PCR product (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
01F_PI3 | tgagaagggtgtgtgaaggaa | PCR and sequencing | 724 | 55 |
01R_PI3 | accactcccagcatcaa | PCR and sequencing | 724 | 55 |
02F_PI3 | gagttttttgcaggaccagg | PCR and sequencing | 717 | 52 |
02R_PI3 | gaacagaaagctgaaatctg | PCR and sequencing | 717 | 50 |
Seq_P13_1328bp_F | caagctggactgcataaaga | PCR | 1328 | 54 |
Seq_P13_1328bp_R | cagccttcttttgtgtcttc | PCR | 1328 | 53 |
Seq_P13_Int1_F | tgcataaagattggtatggc | sequencing | - | 52 |
Seq_PI3_Int2_F | tttaaaccttgggtgtggac | sequencing | - | 54 |
Seq_PI3_Int3_F | gaggtgtaccttccctactc | sequencing | - | 54 |
-1077_A_F | ctctccttgtctcAgtgtattagagtc | gel shift assay | - | - |
-1077_G_F | ctctccttgtctcGgtgtattagagtc | gel shift assay | - | - |
-1067_A_ F | ctcagtgtattagAgtcgtttttctca | gel shift assay | - | - |
-1067_G_F | ctcagtgtattaggGtcgtttttctca | gel shift assay | - | - |
+1063_A_F | gtgtattagagtcAtttttctcagaca | gel shift assay | - | - |
+1063_G_F | gtgtattagagtcGtttttctcagaca | gel shift assay | - | - |
-960_T_F | ggaacccccgtttTcccctttcattactt | gel shift assay | - | - |
-960_D_F | ggaacccccgtttcccctttcattactt | gel shift assay | - | - |
-911_A_F | gttaatagaccagaccaaAtctcacac | gel shift assay | - | - |
-911_G_F | gttaatagaccagaccaaGtctcacac | gel shift assay | - | - |
-689_C_F | tgtatacatgataCatgttttctacta | gel shift assay | - | - |
-689_G_F | tgtatacatgataGatgttttctacta | gel shift assay | - | - |
-675_C_F | atgttttctactaCtttctgattattt | gel shift assay | - | - |
-675_T_F | atgttttctactaTtttctgattattt | gel shift assay | - | - |
-453_T_F | ttgatgctgggagTggtaaaatgataa | gel shift assay | - | - |
-453_G_F | ttgatgctgggagGggtaaaatgataa | gel shift assay | - | - |
-338_G_F | gaataaccttcgGtgattcctttctcttct | gel shift assay | - | - |
-338_A_F | gaataaccttcgAtgattcctttctcttct | gel shift assay | - | - |
-258_A_F | taataagtgagccAgcacttctactct | gel shift assay | - | - |
-258_G_F | taataagtgagccGgcacttctactct | gel shift assay | - | - |