Table 1 SNPs in RAAS pathway genes, their location, primer sequences, PCR conditions and restriction enzyme with product sizes.

*REN

C>T (-4063)

Promoter

F: 5'-AAA CTA GAA TGG GCT ACC AGA-3'

R: 5'-GCT GTG ACT TGT CTC TTC CTG A-3'

235

60°C/Taq I/

C = 163, 68

T = 235

*REN

(ACAG)n

Intron G

F: 5'-ACA GTA CCTT CCC TCC TC TAC TCA-3'

R: 5'-CTC TAT GGA GCT GGT AGA ACC TGA-3'

255

^a1 = 255

2 = 259

3 = 263

4 = 267

5 = 271

*AGT

A>G (-6)

Promoter

F: 5'-CCC TCA GCT ATA AAT AGA GCA TC-3'

R: 5'-GCA GGA AGA CCT GAC CAT CT-3'

308

61°C/Bst NI/

G = 245, 63

A = 36,209,63

*AGT

Glu53Stop

Exon 2

F: 5'-ACC ATC CTC TGC CTC CTG-3'

R: 5'-TCC AAG GCT CCC AGA TAG AG-3'

398

58°C/PvuII/

C = 102, 102, 194

T = 204, 194

*AGT

Thr174Met

Exon 2

F: 5'-CAA TTC AGG CCA AGA CAT CC-3'

R: 5'-GCC AGA GCC AGC AGA GAG-3'

674

58°C/Nco I/

C = 502, 172

T = 256, 246, 172

*AGT

Met235Thr

Exon 2

F: 5'-GAT GCG CAC AAG GTC CTG-3'

R: 5'-CAG GGT GCT GTC CAC ACT GGC TCG C-3'

302

57°C/BstUI/

T = 302

C = 278, 24

ACE

Ins/Del

Intron 16

F: 5'-CTG GAG ACC ACT CCC ATC CTT TCT-3'

R: 5'-GAT GTG GGC ATC ACA TTC GTC AGA T-3'

[Ref: Nucleic Acids Res. 1992, 20: 1433]

490

Ins = 490

Del = 190

*AT1

A>C (-1166)

Promoter

F: 5'-AAG AAG CCT GCA CCA TGT TT-3'

R: 5'-CCA TCT TAC GGG CAT TGT TT-3'

626

58°C/Dde I/

A = 536, 90

C = 119, 417, 90

*AT1

A>T (-777)

Promoter

F: 5'-AGT CAC CCT ACT CAC CTA GCT AAC A-3'

R: 5'-AGA CAT CAC GAG ACT ACA GAT CAA-3'

192

60°C/Alu I/

A = 173 19

T = 192

*CYP11B2

T>C (-344)

Promoter

5'-TGG AGG GTG TAC CTG TGT GTC A-3'

5' GTC CTG CTG GTC TGA GGA TG3'

270

57°C/Hae III/

T = 189, 81

C = 118, 71, 81

*CMA

G>A (-1903)

Promoter

F: 5'-GGA AAT GTGAGC AGA TAG TGC AGT C-3'

R: 5'-AAT CCG GAGCTG GAG AAC TCT TGTC-3'

285

55°C/BstXI/

G = 195, 90

A = 285

*CMA

C>T (-1777)

Promoter

F: 5'-ATA TCA GTG AAA GCA AAC AGC TT-3'

F: 5'-TAA GTG CCT TTC CCA AAT CA-3'

555

60°C/Mnl I/

C = 54, 501

T = 555