Figure 2

Allele-specific TP73 mRNA expression in human brains. In the BanI RFLP autoradiograms the T-allele (uncut) is indicated by the thick marker line and the C-allele (cut) by the thin marker. Additional smaller fragments ran at a lower position and are not shown. The genomic PCR (G) is a control for equal biallelic representation. The RT-PCR in each case was repeated three times from independent aliquots of cDNA (C). Direct sequencing confirms the pronounced allelic expression bias (stronger upper band) in adult brain 110, with the genomic sequence chromatogram serving as a control for biallelic representation. Less pronounced but reproducible biases are seen among the other brains (weaker upper band in RT-PCR products from brain 86).