Figure 3

Check of the integrity of codon 634 with the Bgl I enzyme. DNA samples were amplified using forward and reverse primers shown in figure 1. The PCR product was incubated in the presence (lane 2–5) or absence (lane 1) of Bgl I. The restriction fragments were run in a non-denaturing 10% PAGE. Lane 1: wild type 634 codon without enzyme incubation ; lane 2: wild type 634 codon ; lane 3: mutation in the first base of the 634 codon (T>C); lane 4: mutation in the second base of the 634 codon (G>A); lane 5: mutation in the third base of the 634 codon (C>G). Both alleles are completely cut by the enzyme in lane 2 and 3, which means that at least the second and third bases of the codon are correct. In lane 4 and 5, the product is not completely cut, which reveals that one allele has a mutation at the second or third base of codon 634.