Figure 3
Variations in monocyte expression of FAM134B are associated with SNP rs257174. (A) The position of the SNP-cluster which is in perfect linkage disequilibrium (r2 = 1) with rs257174 in Asian individuals (CHB/JPT) is shown here with respect to the transcripts of the gene FAM134B. This SNP-cluster contains 9 SNPs located ~9 kbp upstream of the transcriptional start site. The position of the tag SNP rs257174 is indicated. Several SNPs of this cluster are located in a region of open chromatin (identified by DNase I Hypersensitivity site analysis of CD14+ monocytes in the Encode Project), suggesting a regulatory role on gene expression. (B) Impact of rs257174 on FAM134B gene expression in Treg, CD4+ T effector cells, B cells and monocytes (same samples as in Figure 2B). FAM134B mRNA content is plotted with reference to rs257174 genotype. Statistical analysis revealed that the polymorphism is associated with FAM134B gene expression in monocytes only (P = 0.0003). Gene expression was measured using the Affymetrix Human Exon 1.0ST Array and data were normalized using the Robust Multichip Average (RMA) at the gene level. P-values for the association were evaluated using one-way ANOVA on log2-transformed data (n = 15).