Figure 1
From: The promoter of miR-663 is hypermethylated in Chinese pediatric acute myeloid leukemia (AML)
The miR-663 promoter is methylated in AML cell lines. (A) Three CpG island regions can be identified in the promoter of miR-663. (B) MSP analysis of the methylation status of miR-663 in leukemia cell lines shows that the promoter is hypermethylated in 5/11 cell lines. M and U represent MSP results using primer sets for methylated and unmethylated miR-663 genes, respectively. (C) BGS results show the rate of CpG methylation is 91.3% in HL-60 cells, 86.2% in MV4-11 cells, and 81.9% in SHI-1 cells compared to 13.0% in CCRF cells, 21.0% in 697 cells, and 25.4% in Jurkat cells. ● methylated cytosines; ○ unmethylated cytosines.