Table 1 Primer sequences and their nucleotide positions on chromosomes 1 and 12 to amplify the breakpoint regions using PCR, and for construction of the expression vectors for the reporter gene assay
primer name | primer location | sequences (5'-3') |
|---|---|---|
Chr1-6 | Chr1:61006139-61006163 | 5'-AGCTTTAACACGGGAGTATAAGGTT-3' |
Chr12-5 | Chr12:85690331-85690355 | 5'-CTAATGGGAGGTAGTTGGCTAAGTT-3' |
Chr1-6-6 | Chr1:61012306-61012330 | 5'-CTGCAGTCAGACAGATCTAAGATCA-3' |
Chr12-5-8 | Chr12:85698309-85698333 | 5'-ACAGTTCTTCTCAAGACAATATGCC-3' |
Chr1-Narrow F | Chr1:61013248-61013272 | 5'-ACTCACTGTGACCCTATGATTTAAG-3' |
Chr12-Narrow F | Chr12:85698739-85698763 | 5'-CCTCCATCTGTTTTATGTAATGCTG-3' |
Chr1-Narrow R | Chr1:61013944-61013964 | 5'-CGGCTGTACACATAGCTACCA-3' |
Chr12-Narrow R | Chr12:85699656-85699675 | 5'-TCATTTATTTGCCCCTTGGA-3' |
Chr12-84222865 F-BamHI | Chr12:85698734-85698753 | 5'-ATAGGATCCCCCAGCCTCCATCTGTTTTA-3 |
Chr1-60785185 R | Chr1:61012577-61012597 | 5'-ATAGGATCTTTGGTCAACAATGACTGCAT-3' |
Chr12-84224235 R | Chr12:85700085-85700104 | 5'-ATAGGATCTTGGGGACAAATTCACAGGT-3' |