Figure 1

Designing AOs to target ESEs in dystrophin exon 25. ESE finder analysis indicates the location of potential binding sites for the splicing factors SF2/ASF, SC35, SRp40 and SRp55 in exon 25 (A). Rescue ESE analysis was employed to gauge the activity of predicted exonic splice enhancers (ESE) (B). The arrow indicates the location of the mutation (c.3385 Insertion A), although this single base insertion made no difference to the splice motifs predicted by ESE Finder (A) or Rescue ESE (B). Antisense oligonucleotide annealing positions are indicated by the solid bars below the sequence (B). The specific sequences and exonic annealing co-ordinates of these AOs can be found in Table 1.