BRCA2 Primers | Nucleotide sequence | Size of PCR products | PCR temperature |
---|---|---|---|
Breakpoint analysis | |||
C2del3-F2 | 5'_CAAGATCACTTCATTGATTTGTGAG_3' | 4572 bp | 59°C |
C2del3-R2 | 5'_CGCTATATTTCTGTGTGCCTTTAAT_3' | ||
Transcript analysis | |||
Fragment analysis | |||
Exon 02-06 | 480 bp | 56°C | |
Rc2-01-F2 | 5 '6FAM_GATCCAAAGAGAGGCCAAC_3' | ||
Rc2-06-R | 5'_CAAACTCCCACATACCACTGA_3' | ||
Exon 02-10 | 1350 bp | 56°C | |
Rc2-01-F2 | 5' 6FAM _GATCCAAAGAGAGGCCAAC_3' | ||
Rc2-10R | 5'_TGGTAGGCTAGAAATACGTGGC_3' | ||
Pyrosequencing | |||
Allelic expression of c.-26G > A | |||
Ex1-2 F | TACTCCGGCCAAAAAAGAACTGCA | ||
Ex2-Ex2/4 R | TATGTCTACTATTGGGAACATTCCTTCCTG | 166 pb | 55°C |
Ex2-Ex3/4 R | ATTGGGAACATTCCTTCCTAAGTCTA | 411 pb | 55°C |
Ex2 R | GATCCAATAGGCATTTTTACCTACGATATTCC | 89 pb | 55°C |
Ex2 SEQ R | TACCTACGATATTCCTCCAATG | ||
Competitive quantitative PCR | |||
EX2 Cpcr F | TGAAATTTTTAAGACACGCTGCAACA | 60°C | |
EX2/3 Cpcr R | AAACCAATTAAGACTTATTGGTCCTAAATCT | 59 bp | 60°C |
EX2/4 Cpcr R | ACTATTGGGAACATTCCTTCCTGCT | 47 bp | 60°C |
EX2 Cpcr SEQ F | TTAAGACACGCTGCAA |