A possible role for miRNA silencing in disease phenotype variation in Swedish transthyretin V30M carriers

BMC Medical Genetics

Table 2 miRNA target predictions for rs62093482

wt (C) allele	Identified with	Predicted binding to wt allele	Predicted binding to polymorphic allele
hsa-miR-593	MicroInspector	RegRNA/RNAhybrid	RegRNA/RNAhybrid
hsa-miR-492	MicroInspector	RNAhybrid	RNAhybrid
hsa-miR-1245	PITA	RNAhybrid	RNAhybrid
hsa-miR-581	PITA	RegRNA/RNAhybrid	RNAhybrid^a
hsa-miR-142-3p	PITA	RegRNA/RNAhybrid	RNAhybrid^a
Polymorphic (T) allele	Identified with	Predicted binding to wt allele	Predicted binding to polymorphic allele
hsa-miR-643	MicroInspector/PITA	RNAhybrid^b	RegRNA/RNAhybrid
hsa-miR-337-3p	MicroInspector	RNAhybrid^b	RegRNA/RNAhybrid
hsa-miR-384	MicroInspector	RegRNA/RNAhybrid	RegRNA/RNAhybrid
hsa-miR-622	PITA		RegRNA/RNAhybrid
hsa-miR-325	PITA	RNAhybrid^b	RegRNA/RNAhybrid

Underlined; predictions unique for wt or polymorphic target respectively in the subsequent validation procedure. In bold, miRNA target predictions that fulfil the criteria. ^aPredicted for polymorphic target with only slightly weaker binding, C:G > U:G. ^bDecreased binding specificity when predicted to wt target leading to reduced thermodynamic properties.

ISSN: 1471-2350