An extension to a statistical approach for family based association studies provides insights into genetic risk factors for multiple sclerosis in the HLA-DRB1 gene

  • Sreeram V Ramagopalan1, 2,

    Affiliated with

    • Roisin McMahon2,

      Affiliated with

      • David A Dyment1, 2,

        Affiliated with

        • A Dessa Sadovnick3,

          Affiliated with

          • George C Ebers1, 2 and

            Affiliated with

            • Knut M Wittkowski4Email author

              Affiliated with

              BMC Medical Genetics200910:10

              DOI: 10.1186/1471-2350-10-10

              Received: 31 December 2008

              Accepted: 04 February 2009

              Published: 04 February 2009

              Abstract

              Background

              Multiple sclerosis (MS) is a complex trait in which genes in the MHC class II region exert the single strongest effect on genetic susceptibility. The principal MHC class II haplotype that increases MS risk in individuals of Northern European descent are those that bear HLA-DRB1*15. However, several other HLA-DRB1 alleles have been positively and negatively associated with MS and each of the main allelotypes is composed of many sub-allelotypes with slightly different sequence composition. Given the role of this locus in antigen presentation it has been suggested that variations in the peptide binding site of the allele may underlie allelic variation in disease risk.

              Methods

              In an investigation of 7,333 individuals from 1,352 MS families, we assessed the nucleotide sequence of HLA-DRB1 for any effects on disease susceptibility extending a recently published method of statistical analysis for family-based association studies to the particular challenges of hyper-variable genetic regions.

              Results

              We found that amino acid 60 of the HLA-DRB1 peptide sequence, which had previously been postulated based on structural features, is unlikely to play a major role. Instead, empirical evidence based on sequence information suggests that MS susceptibility arises primarily from amino acid 13.

              Conclusion

              Identifying a single amino acid as a major risk factor provides major practical implications for risk and for the exploration of mechanisms, although the mechanism of amino acid 13 in the HLA-DRB1 sequence's involvement in MS as well as the identity of additional variants on MHC haplotypes that influence risk need to be uncovered.

              Background

              Multiple sclerosis (MS) is a common inflammatory disease of the central nervous system characterized by myelin loss, axonal pathology and progressive neurological dysfunction [1]. The aetiology of MS is unknown, but it is clear that both genetic and environmental components are important [2].

              The only consistent genetic association with MS in Northern Europeans had been with extended MHC haplotypes especially those containing HLA-DRB1*1501 [3]. Recently, the interleukin 7 receptor (IL7R) interleukin 2 receptor (IL2R), ecotropic viral integration site 5 (EVI5) [4] and kinesin family member 1B (KIF1B) [5] genes have been shown to be additional MS susceptibility loci [3, 6, 7]. However, the MHC is the largest contributor to MS risk [3].

              The association between MS and MHC class II has been fine mapped to the extended haplotype HLA-DQA1*0102-DQB1*0602-DRB1*1501-DRB5*0101 [8]. Intense linkage disequilibrium within the MHC has prevented the exact susceptibility locus from being identified. Analysis of the MHC region with a large number of markers as well as classical typing show evidence for the involvement of the class II region only [9, 10]. The paradigm is more complex than one in which the HLA-DRB1*15 allele acts solely to increase MS risk. Our previous investigations have shown that HLA-DRB1*15 and HLA-DRB1*17 bearing haplotypes increase risk of MS, while HLA-DRB1*14 and HLA-DRB1*11 bearing haplotypes are protective [11, 12]. Additionally, HLA-DRB1*10, DRB1*01, and DRB1*08 interact with HLA-DRB1*15 to influence disease risk [11, 12].

              MHC class II molecules present antigen to CD4+ T helper cells and are integral to successful maintenance of self tolerance by the immune system and the adaptive immune response to invading pathogens [13]. Each HLA-DRB1 allele forms, by the presence of defined amino acid anchors, a number of specific pockets comprising a peptide binding groove [14]. Different HLA-DRB1 alleles may thus have different binding affinities for disease-related peptides as determined by their protein sequence, subsequently influencing composition of T cell repertoires, ultimately resulting in HLA-DRB1 alleles having varying effects on disease risk. However, protein sequence analysis has failed to provide clarity. Class II alleles in MS patients are structurally no different to those in healthy controls [15]. While some studies have suggested that variable residues in the DR beta chain may determine MS susceptibility [1618], others found no evidence that MS pathogenesis is mediated by allele overlapping antigen binding sites [19]. However, these studies were based on a relatively small number of individuals and, thus, may have been underpowered to detect any relevant effects [11, 12]. More recently, Barcellos et al. [20], by aligning the protein sequences of HLA-DRB1*1501, *1503, *1701, *0401, *0801, and *0803 with that of HLA-DRB1*140101, *140102, *140103, and *1404 have suggested that the amino acid at position 60 of the HLA-DRB1 protein sequence determines the effect of a HLA-DRB1 allele on MS susceptibility. This model however does not take into account other HLA-DRB1 resistance alleles, notably HLA-DRB1*11, *10 and *01 [12].

              The HLA-DRB1 gene is unusual in that many loci in the coding sequence can have any one of the four nucleotides depending on the allelotype. Thus, empirical studies were difficult, first, because such variability requires large sample sizes, but – even more importantly – because most traditional statistical methods are limited to the more typical case of bi-allelic loci. We present here the largest systematic investigation to date and an extended more sensitive statistical approach, which, for the first time, will allow us to determine empirically whether or not the nucleotide or protein sequence of HLA-DRB1 can account for allelic susceptibility to MS.

              Methods

              Particpants

              All participants in the study were ascertained through the ongoing Canadian Collaborative Project on the Genetic Susceptibility to MS (CCPGSMS), for which the methodology has been previously described [21].

              Genotyping

              Total genomic DNA, extracted from whole blood as part of the CCPGSMS, was used to type HLA-DRB1 alleles by an allele-specific PCR amplification method [22]. All genotypes were generated blind to pedigree structure and disease status of the individual. Initially, HLA-DRB1 alleles were classified into 10 categories, HLA-DRB1*01 to HLA-DRB1*10. Four of them were then subdivided, *05 into *11/12, *06 into *13/14, *02 into *15/16, and *03 into *17/18. Since then, these "two-digit" genotypes have been refined by adding two or four more digits. The first two digits describe the type, which corresponds to the serological antigen carried by an allelotype. The third and fourth digits are used to list the allele subtypes, numbers being assigned in the order in which the DNA sequences were determined. Alleles whose numbers differ in the first four digits must differ in one or more nucleotide substitutions that change the amino acid sequence of the encoded protein. Alleles that differ only by synonymous nucleotide substitutions within the coding sequence are distinguished by the use of the fifth and sixth digits.

              In our population, 24 PCRs were carried out to amplify allelotypes corresponding to alleles HLA-DRB1*01, HLA-DRB1*04, HLA-DRB1*07, HLA-DRB1*08, HLA-DRB1*09, HLA-DRB1*10, HLA-DRB1*11, HLA-DRB1*12, HLA-DRB1*13, HLA-DRB1*14, HLA-DRB1*15, HLA-DRB1*16, HLA-DRB1*17, and HLA-DRB1*18. Each HLA-DRB1 genotype was scored twice by independent observers.

              Sequence Information

              HLA-DRB1 allele sequence information was obtained from the Immunogenetics database of the European Bioinformatics Institute [23]ftp://​ftp.​ebi.​ac.​uk/​pub/​databases/​imgt/​mhc/​hla/​Alignments_​Rel_​2.​21.​zip, whose nucleotide and amino acid numbering scheme will be used here.

              Statistical Analysis

              For terrestrial life forms, the number of alleles per single nucleotide polymorphism (SNP) is limited to five: A, C, G, T, and X (deletion), although only two alleles have been seen for most human SNPs. For HLA-DRB1 and HLA-DRB2 SNPs, however, up to 4 and 5 nucleotides, respectively, have been observed. As proven in [24], the informative data for each bi-allelic parental mating type (PMT) can be organized into three strata, with two informative filial constellations each:

              Mating            Offspring Genotypes

                 A.A~A.C         A.A   A.C

                 A.C~A.C         A.A   C.C (A.C children are non-informative)

                 A.C~C.C         A.C   C.C

                 ...

                 T.T~T.X         T.T   T.X

                 T.X~T.X         T.T   X.X (T.X children are non-informative)

                 T.X~X.X         T.X   X.X

              With three alleles, only two constellations need to be considered:

              Mating            Offspring Genotypes

                 A.A~C.G         A.C      A.G

                 A.C~A.G         A.A   A.C   A.G   C.G

                 ...

                 X.X~T.G         G.X      T.X

                 X.G~X.T         G.T   G.X   T.X   X.X

              With four or five alleles, each stratum contains four informative filial genotypes, e.g.:

              Mating            Offspring Genotypes

                 A.C~G.T         A.G,   A.T   C.G   C.T

              To determine, whether a particular allele confers risk, children with this allele need to be 'paired' within the same PMT stratum with children having the same other allele. For bi-allelic PMTs, this leaves three informative mating types per combination of alleles, each with two informative filial genotypes [24]. For tri-allelic PMTs, two situations may arise. One homozygous parent, also yields only two filial genotypes, while two heterozygous parents, yield strata with four filial genotypes. For quad-allelic PMTs, both parents are necessarily heterozygous yielding, again, four possible filial genotypes.

              After excluding non-informative children, all filial genotypes within a PMT stratum have the same expectation under the null hypothesis. Thus, for the special case of bi-allelic parents, it has been suggested [25] that the sign/McNemar test for exact ties be applied [26], i.e. to count how often either of the genes were transmitted, irrespective of the PMT. Stratification [24] yields a test statistic based on counts representing independently observed events (cases born, rather than alleles transmitted, which are subject to identical genetical and environmental confounders). Here, the need for stratification is even more apparent than for bi-allelic parents [2729], as there are, for instance, twice as many A alleles to be expected among the children of A.C~A.G parents than either C or G alleles.

              When developing a test suitable for multi-allelic loci, it is useful to note that the sign test can be written in the two equivalent forms given at the ends of the following equation:
              http://static-content.springer.com/image/art%3A10.1186%2F1471-2350-10-10/MediaObjects/12881_2008_Article_424_Equa_HTML.gif

              As http://static-content.springer.com/image/art%3A10.1186%2F1471-2350-10-10/MediaObjects/12881_2008_Article_424_IEq1_HTML.gif and the "standardized residuals" http://static-content.springer.com/image/art%3A10.1186%2F1471-2350-10-10/MediaObjects/12881_2008_Article_424_IEq2_HTML.gif and http://static-content.springer.com/image/art%3A10.1186%2F1471-2350-10-10/MediaObjects/12881_2008_Article_424_IEq3_HTML.gif are symmetric, each follows asymptotically a standard Gaussian distribution, allowing the contributions from different PMT strata to be combined in an additive fashion.

              To test the effect of allele A, for instance, the following strata are informative:

              Mating      Informative Offspring Genotypes

                 A.A~A.x: A.A   vs   A.x

                 A.x~A.x: A.A   vs         x.x

                 A.x~x.x:      A.x      vs   x.y

                 A.x~y.y:      A.y      vs   x.y

                 A.x~A.y: A.A   vs         x.y

                 A.x~x.y:      (A.x + A.y)   vs   (x.x + x.y)

                 A.y~x.y:      (A.x + A.y)   vs   (x.y + y.y)

                 A.x~y.z:      (A.y + A.z)   vs   (x.y + x.z)

              The informative children A.A in the A.x~A.x and A.x~A.y strata differ from the 'non-A' (x.x or x.y) counterparts by two alleles. As in [24], this will be accounted for by assigning twice the weight to these strata, rather than assuming that the effects of the two alleles transmitted to the same child are independently observed.

              With more than two alleles, several comparisons could be made within the A.x~A.y stratum, e.g.,

              Mating      Informative Offspring Genotypes

                 A.x~A.y: (A.A + A.y)   vs   (A.x + x.y)

                 A.x~A.y: (A.A + A.x)   vs   (A.y + x.y)

              In both cases, the term to the left (A.A or A.x/A.y) would have more A alleles than the corresponding term on the right (A.x/A.y or x.y, respectively). Averaging across these comparisons yields the same term (A.x+A.y)/2 on both sides which, as the heterozygous children born to two heterozygous children in the biallelic case, can be ignored, resolving this seeming ambiguity. Again, stratification is essential for developing a sound statistical approach to deal with this complex situation.

              From column card in Figure 1, the maximum number of informative strata for the influence of a given allele is 3 × 4 + 3 × 12 + 6 = 54 out of the 105. Of course, if a particular PMT is not observed, the corresponding strata need not be included. Column w gives the weight to be applied to this stratum's contribution based on the number of alleles differing.
              http://static-content.springer.com/image/art%3A10.1186%2F1471-2350-10-10/MediaObjects/12881_2008_Article_424_Fig1_HTML.jpg
              Figure 1

              Standardized residuals by parental mating type (PMT) with cardinality (card: number of combinations of non-A nucleotides x, y, and z per row) and weight (w: weight, depending on degree of dominance).

              This stratification now allows for different degrees of 'dominance' to be considered. An allele is "dominant" or "recessive", if having a single copy confers the same risk as having two copies or no copy, respectively.

              When both parents are heterozygous for the allele of interest, only those children are counted where both alleles are equal to or different from the allele of interest (n* = n A. A + n ¬A.¬A). Thus, among these strata, the effective sample size depends on the allele considered.

              Using the results of [30], the strata's contributions can be combined into an allele-specific test statistic by forming a single test statistic from the sum of the effect estimates and their variances, respectively, e.g.,
              http://static-content.springer.com/image/art%3A10.1186%2F1471-2350-10-10/MediaObjects/12881_2008_Article_424_Equb_HTML.gif

              where x is the subset of the 54 PMTs informative (relevant and not empty) for nucleotide A, while http://static-content.springer.com/image/art%3A10.1186%2F1471-2350-10-10/MediaObjects/12881_2008_Article_424_IEq4_HTML.gif and http://static-content.springer.com/image/art%3A10.1186%2F1471-2350-10-10/MediaObjects/12881_2008_Article_424_IEq5_HTML.gif states the number of subjects with a preponderance of A or non-A alleles, respectively within the stratum indicated (i.e., n A. A, n A. x, or n A.+, see above).

              From the above, nucleotide-specific test statistics can be obtained by performing the following steps:

              1) Select the PMTs where at least one parent is heterozygous for the nucleotide considered (Figure 1).

              2) Among the strata where both parents are heterozygous for the nucleotide considered, eliminate the counts of children that are also heterozygous for this nucleotide.

              3) By number of parental copies of the nucleotide of interest (1, 2, or 3), count the number of children with more (1 or 2) vs the number of children with less (0 or 1, respectively) alleles of this nucleotide.

              4) Perform the stratified McNemar test [24] based on these six numbers and the weights assigned to each of the three categories of strata according to the degree of dominance to be considered (Figure 1).

              Software

              Statistical analyses were done using S-PLUS 8.0 http://​www.​insightful.​com and the muStat library (available from http://​csan.​insightful and http://​cran.​r-project.​org). Surface and cartoon representations were produced using PyMOL http://​www.​pymol.​org. The program VOLUMES (R. Esnouf., unpublished data) was used with a 1.4 Å radius probe to map the extent of the P4 pocket cavity.

              Results

              Of 7333 genotyped subjects, 3178 children (1773 affected and 1405 unaffected) from 1352 families (1–15 children per family) had complete familial allele-type and disease status information available.

              Sequence analysis identified 93 SNPs that suffice to characterize the differences between the 13 main allelic types of HLA-DRB1. As shown in Table 1, the relationship between nucleotides and allelic groups is complex. Thus, characterizing subjects by the 13 allelic groups (two-digit resolution) may not suffice to identify the genetic factors contributing to disease susceptibility (or resistance). For instance, for the second SNP (nucleotide position (N) 016), HLA-DRB1*04 (T) is uniquely different from all other groups (C), while the third SNP (N037) separates HLA-DRB1*01, *15, and *16 (A) from all other alleles (G). HLA-DRB1*09 is the only allelic group characterized by a single allele at each of the 93 SNPs – all other allelotypes have genetic variability, often with more than two alleles observed within each allelic group at a given SNP. The region between N256 and N308 is characterized by various allelotypes with three or four potential nucleotides per SNP.
              Table 1

              Nucleotides (N#: nucleotide number, #A: amino acid number) found in 94 HLA-DRB1 SNPs discriminating the 13 main two-digit allelic groups (rows).

              Allelotype:

              02

              01

              04

              03

              05

              06

              08

              10

              09

              07

              A#

              N#

              15

              16

                

              17/18

              11

              12

              13

              14

                  

              -25

              14

              A

              A

              A

              A

              G

              G

              G

              G

              G

              G

              G

              A

              A

              -24

              16

              C

              C

              C

              T

              C

              C

              C

              C

              C

              C

              C

              C

              C

              -17

              37

              A

              A

              A

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              -16

              41

              C

              C

              C

              C

              T

              T

              T

              T

              T

              T

              T

              C

              C

              -16

              42

              G

              G

              G

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              -1

              85

              T

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              -1

              86

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              -1

              87

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              4

              97

              C

              C

              C

              C

              A

              A

              A

              A

              A

              A

              A

              C

              C

              4

              98

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              A

              A

              8

              109

              C

              C

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              C

              9

              112

              T

              T

              T

              G

              G

              G

              G

              G

              G

              G

              G

              A

              T

              10

              115

              C

              C

              C

              C

              T

              T

              T

              T

              T

              T

              G

              C

              C

              10

              117

              G

              G

              G

              G

              C

              C

              C

              C

              C

              C

              G

              G

              G

              11

              118

              C

              C

              C

              G

              T

              T

              T

              T

              T

              T

              G

              G

              G

              11

              119

              C

              C

              T

              T

              C

              C

              C

              C

              C

              C

              T

              A

              G

              12

              122

              A

              A

              A

              A

              C

              C

              C

              C

              C

              C

              A

              A

              A

              13

              124

              A

              A

              T

              C

              T

              T

              G

              T

              TG

              G

              T

              T

              T

              13

              125

              G

              G

              T

              A

              C

              C

              G

              C

              CG

              G

              T

              T

              A

              13

              126

              G

              G

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              14

              127

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              A

              14

              129

              G

              G

              A

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              16

              133

              C

              C

              C

              C

              C

              C

              T

              C

              CT

              T

              C

              C

              C

              19

              144

              T

              T

              T

              C

              T

              T

              T

              T

              T

              T

              C

              C

              C

              26

              164

              T

              T

              T

              T

              AT

              T

              T

              T

              T

              T

              T

              A

              T

              26

              165

              C

              C

              G

              C

              C

              C

              A

              C

              C

              C

              G

              T

              C

              28

              169

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              C

              G

              28

              171

              C

              C

              A

              C

              CG

              C

              G

              CG

              CG

              C

              A

              C

              A

              30

              175

              T

              T

              T

              T

              T

              T

              C

              T

              T

              T

              C

              G

              C

              30

              176

              A

              A

              G

              A

              A

              A

              A

              A

              A

              A

              G

              G

              T

              31

              178

              T

              T

              A

              T

              T

              T

              T

              T

              T

              T

              G

              A

              T

              32

              181

              T

              T

              T

              T

              C

              CT

              C

              CT

              CT

              CT

              C

              T

              T

              33

              184

              A

              A

              A

              C

              A

              A

              A

              A

              A

              A

              A

              A

              A

              34

              189

              G

              G

              A

              A

              G

              AG

              G

              AG

              AG

              AG

              A

              A

              G

              37

              196

              T

              T

              T

              T

              AT

              AT

              CT

              AT

              AT

              T

              T

              A

              T

              37

              197

              C

              C

              C

              AC

              AT

              AT

              T

              AT

              AT

              AT

              A

              A

              T

              38

              199

              G

              G

              G

              G

              G

              G

              C

              G

              G

              G

              G

              G

              G

              38

              200

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              C

              T

              T

              47

              227

              T

              AT

              A

              AT

              AT

              AT

              T

              AT

              AT

              A

              A

              A

              A

              53

              246

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              A

              57

              256

              G

              G

              G

              AG

              G

              G

              G

              AG

              AG

              AG

              G

              G

              G

              57

              257

              A

              A

              A

              AG

              A

              A

              AT

              ATG

              AC

              ATG

              A

              T

              T

              57

              258

              CT

              C

              T

              CT

              T

              T

              CT

              CT

              CT

              CT

              T

              C

              C

              58

              260

              C

              C

              C

              C

              C

              A

              C

              C

              C

              C

              C

              C

              C

              58

              261

              CT

              T

              C

              CTG

              CG

              G

              C

              CT

              CTG

              CG

              C

              C

              C

              60

              265

              T

              T

              T

              T

              T

              T

              T

              T

              CT

              T

              T

              T

              T

              60

              266

              A

              A

              A

              A

              A

              A

              AC

              AC

              A

              A

              A

              C

              C

              60

              267

              A

              A

              A

              A

              A

              A

              A

              A

              A

              A

              A

              A

              A

              67

              286

              AC

              ACT

              AC

              ACT

              C

              ACT

              AT

              ACT

              ACT

              AT

              C

              T

              A

              69

              294

              AG

              A

              G

              AG

              G

              AG

              A

              A

              AG

              A

              G

              G

              G

              70

              295

              CG

              G

              C

              CG

              C

              CG

              G

              G

              CG

              G

              C

              C

              G

              70

              296

              A

              A

              A

              A

              A

              A

              A

              A

              AG

              A

              G

              G

              A

              70

              297

              G

              C

              G

              CG

              G

              CG

              C

              C

              CG

              C

              G

              G

              C

              71

              298

              AG

              A

              AG

              AG

              A

              AG

              A

              AG

              AG

              A

              A

              A

              A

              71

              299

              C

              G

              ACG

              AG

              A

              AG

              G

              AG

              AG

              G

              G

              G

              G

              72

              303

              G

              CG

              G

              G

              G

              G

              CG

              CG

              G

              G

              CT

              G

              G

              73

              305

              C

              C

              C

              C

              G

              C

              C

              C

              C

              C

              C

              C

              G

              74

              307

              G

              G

              G

              CG

              C

              CG

              G

              CG

              CG

              CG

              G

              G

              C

              74

              308

              C

              C

              C

              ACT

              AG

              ACT

              C

              CT

              ACT

              CT

              C

              A

              A

              77

              317

              C

              C

              AC

              AC

              A

              C

              C

              C

              AC

              C

              C

              C

              C

              77

              318

              C

              C

              CT

              C

              CT

              C

              C

              C

              C

              C

              C

              C

              C

              78

              320

              A

              A

              A

              A

              A

              A

              A

              A

              A

              A

              A

              T

              AT

              78

              321

              C

              C

              CT

              C

              C

              CT

              CT

              C

              CT

              C

              C

              G

              CG

              85

              341

              T

              T

              CT

              T

              T

              CT

              CT

              T

              T

              T

              T

              T

              T

              86

              344

              TG

              T

              TG

              TG

              TG

              TG

              G

              TG

              TG

              TG

              TG

              T

              T

              90

              357

              A

              A

              A

              AG

              A

              AG

              A

              AG

              A

              AG

              A

              A

              A

              93

              365

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              A

              C

              95

              372

              C

              C

              T

              C

              C

              C

              C

              C

              C

              C

              T

              C

              C

              96

              373

              C

              C

              G

              T

              C

              C

              C

              C

              C

              C

              C

              C

              C

              96

              374

              A

              A

              A

              A

              A

              A

              A

              A

              A

              A

              A

              A

              A

              96

              375

              A

              A

              G

              T

              T

              T

              T

              T

              T

              T

              A

              T

              T

              98

              379

              A

              A

              A

              G

              A

              A

              A

              A

              A

              A

              A

              G

              G

              101

              388

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              101

              389

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              101

              390

              A

              A

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              104

              397

              T

              T

              T

              G

              T

              T

              T

              T

              T

              T

              T

              G

              G

              106

              405

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              T

              T

              114

              429

              C

              C

              C

              C

              C

              C

              C

              C

              CG

              C

              C

              C

              C

              117

              438

              C

              C

              C

              C

              T

              T

              T

              T

              T

              T

              T

              C

              C

              120

              446

              G

              G

              G

              A

              G

              G

              G

              G

              G

              G

              A

              G

              G

              133

              484

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              133

              485

              T

              T

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              133

              486

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              134

              489

              C

              C

              C

              C

              T

              T

              T

              T

              T

              T

              C

              C

              C

              140

              505

              G

              G

              G

              A

              A

              A

              A

              A

              A

              A

              A

              G

              G

              142

              511

              A

              A

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              142

              512

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              142

              513

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              145

              522

              A

              A

              A

              A

              A

              A

              G

              A

              A

              A

              G

              A

              A

              149

              534

              G

              G

              G

              G

              C

              C

              C

              C

              C

              C

              G

              G

              G

              152

              543

              C

              C

              T

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              166

              583

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              166

              584

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              A

              G

              G

              166

              585

              A

              A

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              169

              594

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              A

              A

              179

              624

              C

              C

              T

              C

              C

              C

              C

              C

              C

              C

              T

              T

              T

              180

              625

              G

              G

              G

              C

              G

              G

              G

              G

              G

              G

              G

              G

              G

              181

              629

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              T

              T

              T

              181

              630

              A

              A

              G

              G

              A

              A

              A

              A

              A

              A

              G

              G

              G

              190

              656

              A

              A

              A

              A

              A

              A

              A

              A

              A

              T

              A

              A

              A

              207

              707

              T

              T

              C

              C

              T

              T

              T

              T

              T

              T

              T

              T

              T

              218

              739

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              T

              232

              783

              A

              A

              A

              A

              A

              A

              A

              A

              A

              A

              C

              A

              A

              234

              789

              C

              C

              C

              C

              G

              G

              G

              G

              G

              C

              C

              C

              C

              #Cases:

               

              3994

              208

              1396

              1982

              1873

              1068

              146

              1519

              243

              408

              80

              98

              1653

              Note that many SNPs have more than two nucleotides and some SNPs have several nucleotides for the same allelic group. For instance, Nucleotide 257 can be either A or T within allelotype *12 and A, T, or G within allelotype *13. Given the high prevalence of the HLA-DRB1*15 (15) allele, the nucleotide found in this group of alleles was used as the reference, unless several nucleotides were found in the alleles constituting the HLA-DRB1*15 group.

              This would render current statistical analysis strategies, comparing one allelic group against all others, highly inefficient. To resolve this conundrum, we extended a recently developed statistical approach that allowed us to assess at each of the 93 loci the association (controlled for population admixture) with each individual nucleotide (A, C, G, T, and deletion). The test statistics for this analysis are shown in Table 2 (left side).
              Table 2

              Test statistics for specific alleles at any of the 93 SNPs increasing (positive) or decreasing (negative) the risk of MS.

               

              #N

              #A

              A

              C

              G

              T

               

              A

              C

              G

              T

               

              1

              14

              -25

              6.723

               

              -6.723

               

              1231

              0.506

               

              -0.506

               

              995

              2

              16

              -24

               

              4.485

               

              -4.485

              747

               

              0.512

               

              -0.512

              593

              3

              37

              -17

              12.563

               

              -12.563

               

              1249

              1.774

               

              -1.774

               

              1018

              4

              41

              -16

               

              6.723

               

              -6.723

              1231

               

              0.506

               

              -0.506

              995

              5

              42

              -16

                

              12.563

              -12.563

              1249

                

              1.774

              -1.774

              1018

              6

              85

              -1

                

              -15.587

              15.587

              1139

                

              -2.985

              2.985

              926

              7

              97

              4

              -6.723

              6.723

                

              1231

              -0.506

              0.506

                

              995

              8

              98

              4

              -3.206

               

              3.206

               

              696

              -1.308

               

              1.308

               

              547

              9

              109

              8

               

              11.817

               

              -11.817

              1311

               

              1.629

               

              -1.629

              1028

              10

              112

              9

              -1.313

               

              -9.607

              9.830

              1340

              0.354

               

              -0.829

              0.776

              1066

              11

              115

              10

               

              6.723

              -1.000

              -6.565

              1245

               

              0.506

              0.316

              -0.562

              1004

              12

              117

              10

               

              -6.565

              6.565

               

              1236

               

              -0.562

              0.562

               

              993

              13

              118

              11

               

              12.563

              -6.002

              -6.565

              1544

               

              1.774

              -1.323

              -0.562

              1245

              14

              119

              11

              -1.313

              7.597

              -2.961

              -6.254

              1360

              0.354

              2.093

              -1.418

              -1.354

              1073

              15

              122

              12

              6.565

              -6.565

                

              1236

              0.562

              -0.562

                

              993

              16

              124

              13

              14.806

              -5.027

              -1.172

              -9.371

              1425

              3.079

              -0.726

              0.884

              -2.514

              1133

              17

              125

              13

              -6.121

              -5.444

              13.465

              -4.346

              1507

              -1.794

              -0.856

              3.240

              -1.205

              1225

              18

              126

              13

                

              15.117

              -15.117

              1171

                

              2.781

              -2.781

              961

              19

              127

              14

              -2.961

               

              2.961

               

              657

              -1.418

               

              1.418

               

              528

              20

              129

              14

              -3.750

               

              3.750

               

              544

              -1.403

               

              1.403

               

              455

              21

              133

              16

               

              1.172

               

              -1.172

              236

               

              -0.884

               

              0.884

              155

              22

              144

              19

               

              -6.002

               

              6.002

              1118

               

              -1.323

               

              1.323

              897

              23

              164

              26

                 

              0.156

              41

              0.730

                

              -0.730

              30

              24

              165

              26

               

              4.739

              -3.887

              -1.313

              663

              1.508

              0.732

              -1.289

              0.354

              531

              25

              169

              28

               

              -1.313

              1.313

               

              58

               

              0.354

              -0.354

               

              32

              26

              171

              28

              -4.609

              4.964

              -1.732

               

              395

              -0.967

              0.981

              0.000

               

              310

              27

              175

              30

               

              -3.790

              -1.313

              4.009

              770

               

              -0.885

              0.354

              0.792

              617

              28

              176

              30

              5.203

               

              -4.158

              -2.961

              1055

              1.941

               

              -1.169

              -1.418

              857

              29

              178

              31

              -4.029

               

              -1.000

              4.158

              605

              -1.277

               

              0.316

              1.169

              505

              30

              181

              32

               

              -1.742

               

              1.742

              309

               

              0.000

               

              0.000

              255

              31

              184

              33

              4.485

              -4.485

                

              747

              0.512

              -0.512

                

              593

              32

              189

              34

              -5.998

               

              5.998

               

              425

              -0.372

               

              0.372

               

              348

              33

              196

              37

                  

              18

                  

              9

              34

              197

              37

              -1.897

              3.130

               

              -2.629

              71

              -0.333

              1.386

               

              -1.361

              58

              35

              199

              38

               

              -2.438

              2.438

               

              88

               

              1.508

              -1.508

               

              44

              36

              200

              38

               

              -1.000

               

              1.000

              36

               

              0.316

               

              -0.316

              40

              37

              227

              47

              -4.743

                

              4.743

              116

              -2.008

                

              2.008

              87

              38

              246

              53

              -2.961

               

              2.961

               

              657

              -1.418

               

              1.418

               

              528

              40

              257

              57

              4.879

                

              -4.879

              208

              3.048

                

              -3.048

              153

              41

              258

              57

               

              -1.121

               

              1.121

              29

               

              -1.291

               

              1.291

              11

              42

              260

              58

              -4.929

              4.929

                

              461

              0.246

              -0.246

                

              390

              43

              261

              58

               

              -0.775

              0.000

              1.134

              9

               

              0.258

              0.000

              -0.577

              9

              45

              266

              60

              2.863

              -2.863

                

              488

              2.066

              -2.066

                

              373

              46

              286

              67

              -0.447

              0.447

                

              5

              -0.577

              0.577

                

              3

              47

              294

              69

              -0.956

               

              0.956

               

              67

              -0.117

               

              0.117

               

              49

              48

              295

              70

               

              2.656

              -2.656

               

              69

               

              0.289

              -0.289

               

              39

              49

              296

              70

              1.877

               

              -1.877

               

              92

              -0.367

               

              0.367

               

              67

              50

              297

              70

               

              -6.363

              6.363

               

              428

               

              -1.261

              1.261

               

              300

              52

              299

              71

              -0.884

              4.447

              -4.196

               

              159

              -0.717

              2.535

              -2.137

               

              96

              54

              305

              73

               

              2.218

              -2.218

               

              1086

               

              1.724

              -1.724

               

              903

              55

              307

              74

               

              -3.558

              3.558

               

              203

               

              -2.571

              2.571

               

              139

              56

              308

              74

              -2.333

              2.333

                

              72

              -1.477

              1.477

                

              55

              57

              317

              77

              -1.191

              1.191

                

              273

              -0.931

              0.931

                

              186

              59

              320

              78

              1.121

                

              -1.121

              39

              -0.426

                

              0.426

              22

              60

              321

              78

               

              2.183

              -2.183

               

              17

               

              0.632

              -0.632

               

              10

              62

              344

              86

                

              -1.414

              1.414

              2

                

              0.000

              0.000

              0

              64

              365

              93

              -1.313

              1.313

                

              58

              0.354

              -0.354

                

              32

              65

              372

              95

               

              3.887

               

              -3.887

              566

               

              1.289

               

              -1.289

              476

              66

              373

              96

               

              6.168

              -3.750

              -4.485

              1085

               

              1.428

              -1.403

              -0.512

              880

              67

              375

              96

              14.920

               

              -3.750

              -12.385

              1386

              2.799

               

              -1.403

              -1.818

              1135

              68

              379

              98

              5.914

               

              -5.914

               

              1106

              1.400

               

              -1.400

               

              887

              69

              390

              101

              15.117

               

              -15.117

               

              1171

              2.781

               

              -2.781

               

              961

              70

              397

              104

                

              -5.914

              5.914

              1106

                

              -1.400

              1.400

              887

              71

              405

              106

               

              3.206

               

              -3.206

              696

               

              1.308

               

              -1.308

              547

              73

              438

              117

               

              6.723

               

              -6.723

              1231

               

              0.506

               

              -0.506

              995

              74

              446

              120

              -4.595

               

              4.595

               

              769

              -0.422

               

              0.422

               

              609

              75

              484

              133

                

              -15.117

              15.117

              1171

                

              -2.781

              2.781

              961

              76

              489

              134

               

              6.565

               

              -6.565

              1236

               

              0.562

               

              -0.562

              993

              77

              505

              140

              -9.607

               

              9.607

               

              1325

              -0.829

               

              0.829

               

              1057

              78

              511

              142

              15.117

               

              -15.117

               

              1171

              2.781

               

              -2.781

               

              961

              79

              522

              145

              2.573

               

              -2.573

               

              121

              -1.342

               

              1.342

               

              82

              80

              534

              149

               

              -6.565

              6.565

               

              1236

               

              -0.562

              0.562

               

              993

              81

              543

              152

               

              3.750

               

              -3.750

              544

               

              1.403

               

              -1.403

              455

              82

              584

              166

              -1.000

               

              1.000

               

              36

              0.316

               

              -0.316

               

              40

              83

              585

              166

              15.117

               

              -15.117

               

              1171

              2.781

               

              -2.781

               

              961

              84

              594

              166

              -3.206

               

              3.206

               

              696

              -1.308

               

              1.308

               

              547

              85

              624

              179

               

              5.203

               

              -5.203

              1003

               

              1.941

               

              -1.941

              816

              86

              625

              180

               

              -4.485

              4.485

               

              747

               

              -0.512

              0.512

               

              593

              87

              629

              181

               

              3.348

               

              -3.348

              718

               

              1.199

               

              -1.199

              581

              88

              630

              181

              7.597

               

              -7.597

               

              1248

              2.093

               

              -2.093

               

              999

              89

              656

              190

              -0.450

                

              0.450

              180

              -0.616

                

              0.616

              132

              90

              707

              207

               

              -6.168

               

              6.168

              1032

               

              -1.428

               

              1.428

              823

              91

              739

              218

               

              2.961

               

              -2.961

              657

               

              1.418

               

              -1.418

              528

              92

              783

              232

              1.000

              -1.000

                

              36

              -0.316

              0.316

                

              40

              93

              789

              234

               

              6.813

              -6.813

               

              1207

               

              0.771

              -0.771

               

              989

              Left side: analysis of cases, right side: analysis of unaffected siblings.

              Traditionally, family-based association studies utilize information from affected children only. With MS, however, the unaffected siblings of cases can be reasonably assumed to have similar genetic and environmental risk factors. Thus, one can analyze the siblings in the same fashion as the controls, after reversing the role of putative protective and risk alleles. If a nucleotide at a particular locus confers a risk, its absence should confer protection. The right side of Table 2 shows that the genetic constellations seen in the siblings, in fact, closely resemble the results seen in the cases, thereby validating the results.

              To be asymptotically equivalent with the TDT [25], the stratified McNemar test [24], by default, assumes a co-dominant model, doubling the weight of the homozygous children born to two heterozygous parents. For dominant and recessive alleles, homozygous children (for the risk allele or the wild type, respectively) carry either no or the same risk, respectively, as heterozygous children, Thus, by assigning zero weight to some and equal weights to other strata, one can shift the power towards recessive and dominant models, respectively. The results under the dominant, but not the recessive model (data not shown) were similar to the results under the co-dominance model, suggesting that individual alleles contribute substantially to risk.

              Based on these results, candidate loci for MS risk are (N = nucleotide position, A = Amino acid position):

              Position            Nucleotide(s)      Allelotype Carrying Risk Nucleotide

              N085:   T   HLA-DRB1*15

              N124-126/A013:   AGG   HLA-DRB1*15/16

              N375/A096:   A   HLA-DRB1*10/15/16

              N390, 485, 511, 585/A101-142:   A, T, A, A   HLA-DRB1*15/16

              Thus, the sequence variants of HLA-DRB1*15 that increase MS risk can be narrowed down to these regions (Figure 2). N085 is in the promoter region, A013 is part of the antigen presentation P4 pocket, while A133-142 are part of the CD4 binding region [29]. (The region from #33 (N196/A37) to #64 (N365/A93) has more variability than the 13 categories can explain.)
              http://static-content.springer.com/image/art%3A10.1186%2F1471-2350-10-10/MediaObjects/12881_2008_Article_424_Fig2_HTML.jpg
              Figure 2

              Position of candidate risk polymorphic residues in HLA-DRB1*1501. a. Cartoon representation of HLA-DRB1 (PDB ID 1BX2) in which the Cα atoms of candidate risk polymorphic residues (Table 2) are represented as small spheres (green). The β1 and β2 domains are labeled. b. Surface and cartoon representation of HLA-DRB! viewed from above the binding groove. The three-dimensional arrangement of the P4 pocket is shown as a grey mesh. The Cα atoms of the polymorphic residues 13 β (green) at the base of the P4 pocket and residue 60 β (orange) are represented as small spheres.

              Discussion

              Multiple sclerosis is unambiguously associated with the MHC class II region and this gene exerts the strongest genetic effect on the risk of developing the disease [3]. We have shown that A013 is the key amino acid in defining the risk of HLA-DRB1, yet no molecular or functional explanation can be given for the dominant-negative effects of *14 and *11, the complementary effects of *08, and the protective nature of *01 and *10 in the presence of *15. It has been speculated that poor engagement of the encephalitogenic peptide in the context of *14 acts to alter the immune response in a dominant-negative manner and thereby reduce the effect of *15 [20]. Another explanation is that *14 binds one or more peptides that can delete autoreactive T cells.

              In this study, we performed the first empirical investigation of the nucleotide sequence of HLA-DRB1 with respect to determining disease risk. The statistical analysis employed in this study differs fundamentally from previous analyses conducted in that it is based on the actual nucleotides at a SNP, rather than HLA allelotypes. In other words, it allows at each locus the cases (or controls) to be categorized differently according to the nucleotides observed. In principle, we might have done the same analysis based on individual sequence data, irrespective of the two- (four- or six-) digit allele categories. In fact, using individual sequence data would avoid the ambiguities which reduced the sample size to fewer than 300 for the SNPs between N196 and N372 (Table 2) due to some allelotypes having many different nucleotides at these loci.

              For amino acid 13, for instance, we count cases with unambiguous parental mating type for the following HLA-DRB1 allelotypes (see Table 3):
              Table 3

               

              Nucleotide

              N124

              N125

              N126

              A:

              15, 16

              04, 07

              -

              C:

              04

              03, 11, 13

              -

              G:

              12, 08

              15, 16, 08

              15, 16

              T:

              01, 03, 11, 13, 10, 09, 07

              01, 10, 09

              01, 04, 03, 11, 12, 13, 14, 08, 10, 09, 07d

              ambiguous:

              14

              14

               

              (numbers indicate HLA-DRB1 allelotype)

              As the categories are refined or individual sequence data becomes available, ambiguous cases will become fewer or avoided altogether, respectively.

              As in the case of case-control studies [31], counting alleles transmitted to cases, as suggested by [25] in the widely used Transmission-Disequilibrium-Test (TDT) has recently been proven to result in a suboptimal analysis strategy even in the case of bi-allelic parents [24], because the TDT's variance estimate is based, in part, on the counts of non-informative heterozygous children born to two heterozygous parents. As with Student's t- vs. the Gauss' z-test (which are also asymptotically equivalent), this additional variance would need to be accounted for. The stratified McNemar test [24], in contrast, avoids this problem by replacing 2 × (n P. P + n P. Q + n Q. Q) in the denominator by 4 × (n P. P + n Q. Q). For tri- or tetra-allelic parents, stratification is even more important, because it also leads to novel analysis strategies.

              Barcellos et al. [20] have suggested that amino acid 60 accounts for the protective effect of the HLA-DRB1*14 allele, because this locus is close to the P4 binding pocket and HLA-DRB1*1401 encodes histidine (CAC), while HLA-DRB1*1701, *0401, *0801 and *1501 all encode tyrosine (TAC). Upon close inspection of the SNP profiles, however, the observed relationship between HLA-DRB1*15/*15, HLA-DRB1*15/*08 and HLA-DRB1*14 seems to be more complex.

              Among the 4-digit HLA-DRB1 allelotypes, the majority of alleles code TAC for tyrosine, as does HLA-DRB1*15 (all 4-digit allelotypes).

              Codon      Four digitHLA-DRB1*14allelotype carrying codon

                 TAC (14xx): 02,03,05,06,09,11,12,13,14,15,17,18,19,20,21,23,24,27,29,30,33,

                       36,37,40,41,42,43,44,45,36,47,51,52,56,57,59,63,65,67,76,77

                 CAC (14xx): 01,04,07,08,10,16,22,25,26,28,31,32,34,35,38,39,49,50,53,54,55,

                       58,60,61,62,68,69,70,71,72,73,74,75

                 TCC (14xx): 48,64

              In the population of [32], 30% of the population were genotyped at the 4-digit level. Among them, 82% were *1401 and 9% were *1404. If one assumes that these 30% were representative and that the same proportion holds for our population, H at P60 could exert its "protective" effect in about 90% of all 14xx subjects. However, the purported disease risk increasing TAC codon is also seen in *0101, *0103, *0104, *0110, and *0111. Thus, if this amino acid should cause *08 to have its special role in *15/*08 heterozygous subjects, this effect should be seen with other alleles, yet this is not the case [11, 12].

              For amino acid 13, instead, *15 (all K) is disjoint from both *08 (all G/glycine) and *14 (mostly S/serine)

              Codon      HLA-DRB1allelotype carrying codon

                 AGG/K (15xx): (all)

                 GGT/G (08xx): (all)

                 TCT/S (14xx): 01,02,03,05,06,07,08,09,12,13,14,16,17,18,19,20,21,22,23,24,25,

                       26,27,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,51,

                       53,54,55,56,58,59,60,62,63,64,65,66,67,69,70,72,74,75,77

                 GGT/G (14xx): 04,11,15,28,29,30,50,52,61,68,71,73,76,

                 CAT/H (14xx): 10,31,57

              From this data, it seems unlikely that histidine at amino acid 60 contributes substantially to the protective effect of this haplotype. Instead, amino acid 13 emerges as a more likely explanation for a disease association gradient [32]. It is the only amino acid that shows sufficient variation to explain a hierarchy of disease associated alleles, raising the possibility that TCT/S is, in fact, associated with protection, while GTT/G is associated with increased risk. Being located right in the center of the P4 binding pocket, amino acid 13 is a residue that is potentially in contact with presented peptides [29, 32]. Another interesting finding of this paper is the implication that the promoter region, may play an important role. This finding lends notion to the idea of regulatory variants contributing to MS risk. As we have previously shown [33], HLA-DRB1 (and therefore amino acid 13) cannot fully explain the MHC class II associated MS risk and these nearby variants remain to be uncovered.

              It has been argued that "low resolution allele grouping [...] maximize [s] statistical power" [20', p. 2821] when comparing one group against all others. When increasing the sample size comes at the expense of increasing within group variance, however, statistical power often suffers. Let us assume that group *02 had not been separated into *15 (3994 cases) and *16 (208) cases. Then, according to Table 4, 208 'G' cases would have been added to the 3,994 'T' cases to a total of 4202, but the size of the 'G' group would have been reduced from 10,674 to 10,466. Even though the number of informative trios would slightly increase (from 1139 to 1171, see Table 2), the test statistic would drop from 11.022 to 10.689. The proposed statistical test, in contrast, utilized the information obtained through high resolution allelotyping (or sequencing) to increase group sizes by combining allelotypes with the same nucleotide at a given locus.
              Table 4

              Variation of genotypes for the amino acids marked in Table 2.

              Allelotype:

              02

              01

              04

              03

              05

              06

              08

              10

              09

              07

              A#

              N#

              15

              16

                

              17/18

              11

              12

              13

              14

                  

              -1

              85

              T

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              -1

              86

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              -1

              87

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              13

              124

              A

              A

              T

              C

              T

              T

              G

              T

              TG

              G

              T

              T

              T

              13

              125

              G

              G

              T

              A

              C

              C

              G

              C

              CG

              G

              T

              T

              A

              13

              126

              G

              G

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              60

              265

              T

              T

              T

              T

              T

              T

              T

              T

              CT

              T

              T

              T

              T

              60

              266

              A

              A

              A

              A

              A

              A

              AC

              AC

              A

              A

              A

              C

              C

              60

              267

              A

              A

              A

              A

              A

              A

              A

              A

              A

              A

              A

              A

              A

              96

              374

              A

              A

              A

              A

              A

              A

              A

              A

              A

              A

              A

              A

              A

              96

              375

              A

              A

              G

              T

              T

              T

              T

              T

              T

              T

              A

              T

              T

              101

              388

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              101

              389

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              101

              390

              A

              A

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              133

              484

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              133

              485

              T

              T

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              133

              486

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              142

              511

              A

              A

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              142

              512

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              T

              142

              513

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              166

              583

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              C

              166

              584

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              A

              G

              G

              166

              585

              A

              A

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              G

              #Cases:

               

              3994

              208

              1396

              1982

              1873

              1068

              146

              1519

              243

              408

              80

              98

              1653

              (Rare observations, such as TCC in only two out of 77 14xx subcategories are omitted)

              As we have demonstrated above, introducing the concept of stratification by parental mating type has not only quantitative advantages [24] over the original more simplistic approach, but also qualitatively different results for SNPs with more than two alleles in the population. As more data are collected, the proposed method could even be extended to address the difference between association (in trans) and protein function (in cis). To do so, one could categorize filial genotypes by pairs of amino acids, rather than nucleotides, so that differences in nucleotides coding for the same protein would be ignored. Of course, to ensure that genetic confounders are eliminated, parental mating types should still be defined based on the genetic code, rather than the amino acid for which it is coding. While this strategy is based on a large number of possible combinations between three-nucleotide parental mating types and combinations of filial amino acids, the number of combinations observed at each locus is likely to be substantially smaller.

              Conclusion

              In conclusion, an extended statistical approach allowed us to identify A013 at the center of the P4 pocket of HLA-DRB1 as a potentially important (although unlikely exclusive) risk factor for MS.

              Declarations

              Acknowledgements

              The authors would like to thank all patients who generously participated in this study and physicians participating in the CCPGSMS. Experiments performed for this investigation comply with current guidelines and ethics. This work was funded by the Multiple Sclerosis Society of Canada Scientific Research Foundation and the Multiple Sclerosis Society of the United Kingdom. SVR is funded by the Medical Research Council of the United Kingdom. The project was also supported in part by Grant Number UL1RR024143 from the U.S. National Center for Research Resources (NCRR). The funding bodies had no role in the design of this study, the collection, analysis, and interpretation of data, the writing of the manuscript and in the decision to submit the manuscript for publication.

              Authors’ Affiliations

              (1)
              Department of Clinical Neurology, University of Oxford, The West Wing, The John Radcliffe Hospital
              (2)
              Wellcome Trust Centre for Human Genetics, University of Oxford
              (3)
              Department of Medical Genetics and Faculty of Medicine, Division of Neurology, University of British Columbia
              (4)
              Center for Clinical and Translational Science, The Rockefeller University

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              34. Pre-publication history

                1. The pre-publication history for this paper can be accessed here:http://​www.​biomedcentral.​com/​1471-2350/​10/​10/​prepub

              Copyright

              © Ramagopalan et al. 2009

              This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.